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2D PAGE: Sample Preparation and Fractionation pp 373–396Cite as

Glycoprotein Enrichment Through Lectin Affinity Techniques

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 424))

Summary

Posttranslational modifications (PTM) of proteins are among the key biological regulators of function, activity, localization, and interaction. The fact that no more than 30,000–50,000 proteins are encoded by the human genome underlines the importance of posttranslational modifications in modulating the activities and functions of proteins in health and disease. With approximately 50% of all proteins now considered to be glycosylated, its physiological importance in mammalian systems is imperative. Aberrant glycosylation has now been recognized as an attribute of many mammalian diseases, including hereditary disorders, immune deficiencies, neurodegenerative diseases, cardiovascular conditions, and cancer. As many potential disease biomarkers may be glycoproteins present in only minute quantities in tissue extracts and physiological fluids, glycoprotein isolation and enrichment may be critical in a search for such biomarkers. For decades, efforts have been focused on the development of glycoprotein enrichment from complex biological samples. Logically, the great majority of these enrichment methodologies rely on the use of immobilized lectins, which permit selective enrichment of the pools of glycoproteins for proteomic/glycomic studies. In this chapter, lectin affinity chromatography in different formats are described, including tubes; packed columns, and microfluidic channels.

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Acknowledgements

This work was supported by Grant No. GM24349 from the National Institute of General Medical Sciences, U.S. Department of Health and Human Services and a center grant from the Indiana 21st Century Research and Technology Fund. This work was also supported by the National Institute of Health through the National Center for Research Resources (NCRR) by grant number RR018942 for the National Center for Glycomics and Glycoproteomics.

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Authors and Affiliations

  1. Indiana University, Bloomington, Indiana

    Yehia Mechref, Milan Madera & Milos V Novotny

Authors
  1. Yehia Mechref
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  2. Milan Madera
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  3. Milos V Novotny
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Editors and Affiliations

  1. Bio-Rad Laboratories GmbH, Munich, Germany

    Anton Posch

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Mechref, Y., Madera, M., Novotny, M.V. (2008). Glycoprotein Enrichment Through Lectin Affinity Techniques. In: Posch, A. (eds) 2D PAGE: Sample Preparation and Fractionation. Methods in Molecular Biology™, vol 424. Humana Press. https://doi.org/10.1007/978-1-60327-064-9_29

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  • DOI: https://doi.org/10.1007/978-1-60327-064-9_29

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-722-8

  • Online ISBN: 978-1-60327-064-9

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