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Meiosis

Volume 1, Molecular and Genetic Methods

  • Book
  • © 2009

Overview

Editors:
  1. Scott Keeney

    1. Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, U.S.A.

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  • State-of-the-art techniques for analysis of meiotic recombination
  • Comprehensive methods for studying meiosis in human and in many commonly used model organisms
  • Chapters on background theory, methodology, and statistical considerations for classical genetic analysis of recombination and crossover interference
  • Cutting-edge methods for the direct detection, quantification, and characterization of the DNA intermediates and products of the recombination pathway

Part of the book series: Methods in Molecular Biology (MIMB, volume 557)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Genetic Methods for Studying Meiotic Recombination and Chromosome Dynamics

    1. Front Matter

      Pages 1-1
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    2. Interaction of Genetic and Environmental Factors in Saccharomyces cerevisiae Meiosis: The Devil is in the Details

      • Victoria E. Cotton, Eva R. Hoffmann, Mohammed F.F. Abdullah, Rhona H. Borts
      Pages 3-20

    3. Optimizing Sporulation Conditions for Different Saccharomyces cerevisiae Strain Backgrounds

      • Susan L. Elrod, Sabrina M. Chen, Katja Schwartz, Elizabeth O. Shuster
      Pages 21-26

    4. Modulating and Targeting Meiotic Double-Strand Breaks in Saccharomyces cerevisiae

      • Alain Nicolas
      Pages 27-33

    5. Methods for Analysis of Crossover Interference in Saccharomyces cerevisiae

      • Franklin W. Stahl, Elizabeth A. Housworth
      Pages 35-53

    6. Measurement of Spatial Proximity and Accessibility of Chromosomal Loci in Saccharomyces cerevisiae Using Cre /loxP Site-Specific Recombination

      • Doris Lui, Sean M. Burgess
      Pages 55-63

    7. Genetic Analysis of Meiotic Recombination in Schizosaccharomyces pombe

      • Gerald R. Smith
      Pages 65-76

    8. Analysis of Meiotic Recombination in Caenorhabditis elegans

      • Kenneth J. Hillers, Anne M. Villeneuve
      Pages 77-97

    9. Visual Markers for Detecting Gene Conversion Directly in the Gametes of Arabidopsis thaliana

      • Luke E. Berchowitz, Gregory P. Copenhaver
      Pages 99-114

  3. Molecular Analysis of Recombination and Protein-DNA Interactions During Meiosis

    1. Front Matter

      Pages 115-115
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    2. Gel Electrophoresis Assays for Analyzing DNA Double-Strand Breaks in Saccharomyces cerevisiae at Various Spatial Resolutions

      • Hajime Murakami, Valérie Borde, Alain Nicolas, Scott Keeney
      Pages 117-142

    3. Genome-Wide Mapping of Meiotic DNA Double-Strand Breaks in Saccharomyces cerevisiae

      • Cyril Buhler, Robert Shroff, Michael Lichten
      Pages 143-164

    4. Detection of Meiotic DNA Breaks in Mouse Testicular Germ Cells

      • Jian Qin, Jaichandar Subramanian, Norman Arnheim
      Pages 165-181

    5. End-Labeling and Analysis of Spo11-Oligonucleotide Complexes in Saccharomyces cerevisiae

      • Matthew J. Neale, Scott Keeney
      Pages 183-195

    6. Detection of SPO11-Oligonucleotide Complexes from Mouse Testes

      • Jing Pan, Scott Keeney
      Pages 197-207

    7. Stabilization and Electrophoretic Analysis of Meiotic Recombination Intermediates in Saccharomyces cerevisiae

      • Steve D. Oh, Lea Jessop, Jessica P. Lao, Thorsten Allers, Michael Lichten, Neil Hunter
      Pages 209-234

    8. Using Schizosaccharomyces pombe Meiosis to Analyze DNA Recombination Intermediates

      • Randy W. Hyppa, Gerald R. Smith
      Pages 235-252

    9. Analysis of Chromatin Structure at Meiotic DSB Sites in Yeasts

      • Kouji Hirota, Tomoyuki Fukuda, Takatomi Yamada, Kunihiro Ohta
      Pages 253-266

    10. Analysis of Protein–DNA Interactions During Meiosis by Quantitative Chromatin Immunoprecipitation (qChIP)

      • Marco Antonio Mendoza, Silvia Panizza, Franz Klein
      Pages 267-283
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Keywords

About this book

Each generation in a sexually reproducing organism such as a fly or a mouse passes through the bottleneck of meiosis, which is the specialized cell division that gives rise to haploid reproductive cells (sperm, eggs, spores, etc. ). The principal function of meiosis is to reduce the genome complement by half, which is accomplished through sequential execution of one round of DNA replication followed by two rounds of chromosome segregation. Within the extended prophase between DNA replication and the first meiotic division in most organisms, homologous maternal and paternal chromosomes pair with one another and undergo homologous recombination, which establishes physical connections that link the homologous chromosomes until the time they are separated at anaphase I. Recombination also serves to increase genetic diversity from one generation to the next by breaking up linkage groups. The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been an explosion of new information about how meiotic chromosomes pair, recombine, and are segregated. Progress has been driven by advances in three main areas: (1) genetic identification of meiosis-defective mutants and cloning of the genes involved; (2) development of direct physical assays for DNA intermediates and products of recombination; and (3) increasingly sophisticated cy- logical methods that describe chromosome behaviors and the spatial and temporal patterns by which specific proteins associate with meiotic chromosomes.

Editors and Affiliations

  • Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, U.S.A.

    Scott Keeney

Bibliographic Information

  • Book Title: Meiosis

  • Book Subtitle: Volume 1, Molecular and Genetic Methods

  • Editors: Scott Keeney

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-527-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-934115-66-4Published: 20 July 2009

  • Softcover ISBN: 978-1-4939-6157-3Published: 23 August 2016

  • eBook ISBN: 978-1-59745-527-5Published: 16 October 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 365

  • Topics: Cell Biology, Microbiology, Eukaryotic Microbiology

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