Overview
- Provides an easily accessible reference volume for genetic modification of hematopoietic cells using various state-of-the-art vector systems
- Comprehensive guide on functional, phenotypic and genotypic screening of gene-modified cells written by leading researchers in the field
- Contains helpful insights into preclinical data acquisition and documentation for gene therapy, including regulatory comments from the EU and US perspectives
- Covers a wide range of cell-based and animal assay formats and systems
Part of the book series: Methods in Molecular Biology (MIMB, volume 506)
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Table of contents (32 protocols)
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Front Matter
Keywords
About this book
Reviews
From the reviews:
"This book provides a unique and comprehensive resource of protocols for the genetic modification of various hematopoietic cell types and up-to-date procedures for molecular and systemic monitoring. … This book is a great help for the design of excellent research in basic hematology, oncology, genetics, and immunology, and also promote the implementation of investigator-driven clinical studies using gene-modified hematopoietic cells. The book represents an outstanding work, which should not be missed in all biomedical research laboratories dealing with gene therapy of hematopoietic cells." (C. Altaner, Neoplasma, June, 2009)
"This book provides a unique and comprehensive resource of protocols for the genetic modification of various hematopoietic cell types and up-to-date procedures for molecular and systemic monitoring. … This book is a great help for the design of excellent research in basic hematology, oncology, genetics, and immunology, and also promote the implementation of investigator-driven clinical studies using gene-modified hematopoietic cells. The book represents an outstanding work, which should not be missed in all biomedical research laboratories dealing with gene therapy of hematopoietic cells." (C. Altaner, Neoplasma, April, 2009)
“Haematopoietic stem cells are attractive targets for gene therapy. … Each chapter provides a brief review of the area, followed by a step-by-step guide to undertaking experiments and includes details of reagents required and a list of useful notes or comments of issues that may arise. Comprehensive reference citations provide sources of further information when required. The book should provide a road map for researchers, clinical investigators and regulators involved in modifying haematopoietic cells.” (Waseem Qasim, Human Genetics, Vol. 128, May, 2010)
Editors and Affiliations
Bibliographic Information
Book Title: Genetic Modification of Hematopoietic Stem Cells
Book Subtitle: Methods and Protocols
Editors: Christopher Baum
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-409-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-58829-980-2Published: 19 December 2008
Softcover ISBN: 978-1-61737-866-9Published: 20 January 2011
eBook ISBN: 978-1-59745-409-4Published: 26 December 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XXII, 490
Number of Illustrations: 74 b/w illustrations, 10 illustrations in colour
Topics: Biochemistry, general, Cell Biology, Stem Cells, Gene Therapy