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Quantification of Strigolactones

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Plant and Food Carotenoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2083))

Abstract

Strigolactones (SLs) are a family of natural products produced by the plants as shoot branching factors and responsible for the induction of hyphal branching in arbuscular mycorrhizal (AM) fungi. They have been also used by parasitic plant seeds as stimulators of their germination as a strategy to ensure the presence of a host in the environment. For all these bioactivities, SLs have kept the attention of the researchers in the last years, increasing the number of published papers, and have opened new areas of research in the multiple roles that they play in the rhizosphere and as plant hormones. However, the low amount of them produced by plants and their rapid degradability make it crucial to develop fast analytical methods with very low limits of quantification. Herein, it is described a protocol for the development of an LC-MS/MS method for the quantification of SLs, using GR24 as IS, in roots exudates and extracts.

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Acknowledgments

This research was supported by the Ministerio de Economía, Industria y Competitividad (MINEICO) (Project AGL2017-88-083-R).

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Authors and Affiliations

  1. Allelopathy Group, Department of Organic Chemistry, Institute of Biomolecules (INBIO), School of Sciences, University of Cadiz, Cadiz, Spain

    Carlos Rial, Rosa M. Varela, José M. G. Molinillo, Alexandra G. Durán & Francisco A. Macías

Authors
  1. Carlos Rial
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  2. Rosa M. Varela
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  3. José M. G. Molinillo
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  4. Alexandra G. Durán
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  5. Francisco A. Macías
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Corresponding author

Correspondence to Francisco A. Macías .

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Editors and Affiliations

  1. CSIC-IRTA-UAB-UB, Centre for Research in Agricultural Genomics (CRAG), Barcelona, Spain

    Manuel Rodríguez-Concepción

  2. Institute for Biology II, University of Freiburg, Freiburg, Germany

    Ralf Welsch

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Rial, C., Varela, R.M., Molinillo, J.M.G., Durán, A.G., Macías, F.A. (2020). Quantification of Strigolactones. In: Rodríguez-Concepción, M., Welsch, R. (eds) Plant and Food Carotenoids. Methods in Molecular Biology, vol 2083. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9952-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9952-1_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9951-4

  • Online ISBN: 978-1-4939-9952-1

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