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Click Chemistry Conjugations

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Antibody-Drug Conjugates

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2078))

Abstract

Click chemistry has found wide application in bioconjugation, enabling control over the site of modification in biomolecules. Demonstrations of this chemistry to construct chemically defined antibody–drug conjugates (ADCs) have increased in recent years, following studies that support benefits of homogeneity and site-specificity of drug placement on the antibody. In this chapter, a brief history of early applications of this chemistry in ADCs is presented. Examples of click chemistries that are utilized for ADC synthesis, including those currently undergoing clinical investigations, are enumerated. Protocols for two common conjugation methods based on carbonyl-aminooxy coupling and strain-promoted azide–alkyne cycloaddition are described.

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  1. Department of Chemistry, Binghamton University, State University of New York, Binghamton, NY, USA

    Tak Ian Chio & Susan L. Bane

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  1. Department of Pharmacy and Pharmaceutical Sciences, Binghamton University, Binghamton, NY, USA

    L. Nathan Tumey

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Chio, T.I., Bane, S.L. (2020). Click Chemistry Conjugations. In: Tumey, L. (eds) Antibody-Drug Conjugates. Methods in Molecular Biology, vol 2078. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9929-3_6

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