Abstract
Capsid-based virus particles are widely engineered as viral nanoparticles and virus-like nanoparticles. The highly organized and uniform capsid structures make them ideal candidates for both in vitro and in vivo applications such as therapeutic delivery vehicles or enzymatic nanoreactors. Viruses have adapted to naturally infect a wide variety of organisms making their production achievable in various expression systems from bacterial to plants. Viral capsids can be modified externally and internally to suit the final application. The wide range of possible applications, ease of production in the system of choice, and customizable modification of viral capsids makes them an attractive choice in the field of nanotechnology. In this chapter we aim to provide a generic protocol for the purification and characterization of virus-derived nanoparticles and methodology for chemically labelling them to monitor their uptake in mammalian cells.
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References
Jordan PC, Patterson DP, Saboda KN, Edwards EJ, Miettinen HM, Basu G, Thielges MC, Douglas T (2016) Self-assembling biomolecular catalysts for hydrogen production. Nat Chem 8(2):179–185. https://doi.org/10.1038/nchem.2416
Young M, Willits D, Uchida M, Douglas T (2008) Plant viruses as biotemplates for materials and their use in nanotechnology. Annu Rev Phytopathol 46:361–384. https://doi.org/10.1146/annurev.phyto.032508.131939
Shukla S, Steinmetz NF (2015) Virus-based nanomaterials as positron emission tomography and magnetic resonance contrast agents: from technology development to translational medicine. Wiley Interdiscip Rev Nanomed Nanobiotechnol 7(5):708–721. https://doi.org/10.1002/wnan.1335
Lizotte PH, Wen AM, Sheen MR, Fields J, Rojanasopondist P, Steinmetz NF, Fiering S (2015) In situ vaccination with cowpea mosaic virus nanoparticles suppresses metastatic cancer. Nat Nanotechnol 11:295. https://doi.org/10.1038/nnano.2015.292
Carrillo-Tripp M, Shepherd CM, Borelli IA, Venkataraman S, Lander G, Natarajan P, Johnson JE, Brooks CL, Reddy VS (2009) VIPERdb(2): an enhanced and web API enabled relational database for structural virology. Nucleic Acids Res 37(Database issue):D436–D442. https://doi.org/10.1093/nar/gkn840
Lomonossoff GP, Evans DJ (2014) Applications of plant viruses in bionanotechnology. In: Palmer K, Gleba Y (eds) Plant viral vectors. Springer, Berlin, pp 61–87. https://doi.org/10.1007/82_2011_184
Catrice EV, Sainsbury F (2015) Assembly and purification of polyomavirus-like particles from plants. Mol Biotechnol 57(10):904–913. https://doi.org/10.1007/s12033-015-9879-9
Saunders K, Sainsbury F, Lomonossoff GP (2009) Efficient generation of cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants. Virology 393(2):329–337. https://doi.org/10.1016/j.virol.2009.08.023
Patterson DP, LaFrance B, Douglas T (2013) Rescuing recombinant proteins by sequestration into the P22 VLP. Chem Commun 49(88):10412–10414. https://doi.org/10.1039/c3cc46517a
Brillault L, Jutras PV, Dashti N, Thuenemann EC, Morgan G, Lomonossoff GP, Landsberg MJ, Sainsbury F (2017) Engineering recombinant virus-like nanoparticles from plants for cellular delivery. ACS Nano 11(4):3476–3484. https://doi.org/10.1021/acsnano.6b07747
Wen AM, Shukla S, Saxena P, Aljabali AAA, Yildiz I, Dey S, Mealy JE, Yang AC, Evans DJ, Lomonossoff GP, Steinmetz NF (2012) Interior engineering of a viral nanoparticle and its tumor homing properties. Biomacromolecules 13(12):3990–4001. https://doi.org/10.1021/bm301278f
Brumfield S, Willits D, Tang L, Johnson JE, Douglas T, Young M (2004) Heterologous expression of the modified coat protein of Cowpea chlorotic mottle bromovirus results in the assembly of protein cages with altered architectures and function. J Gen Virol 85(Pt 4):1049–1053. https://doi.org/10.1099/vir.0.19688-0
Ashley CE, Carnes EC, Phillips GK, Durfee PN, Buley MD, Lino CA, Padilla DP, Phillips B, Carter MB, Willman CL, Brinker CJ, Caldeira JC, Chackerian B, Wharton W, Peabody DS (2011) Cell-specific delivery of diverse cargos by bacteriophage MS2 virus-like particles. ACS Nano 5(7):5729–5745. https://doi.org/10.1021/nn201397z
O’Neil A, Reichhardt C, Johnson B, Prevelige PE, Douglas T (2011) Genetically programmed in vivo packaging of protein cargo and its controlled release from bacteriophage P22. Angew Chem Int Ed 50(32):7425–7428. https://doi.org/10.1002/anie.201102036
Rhee J-K, Hovlid M, Fiedler JD, Brown SD, Manzenrieder F, Kitagishi H, Nycholat C, Paulson JC, Finn MG (2011) Colorful virus-like particles: fluorescent protein packaging by the Qβ capsid. Biomacromolecules 12(11):3977. https://doi.org/10.1021/bm200983k
Sasnauskas K, Buzaite O, Vogel F, Jandrig B, Razanskas R, Staniulis J, Scherneck S, Kruger DH, Ulrich R (1999) Yeast cells allow high-level expression and formation of polyomavirus-like particles. Biol Chem 380(3):381–386. https://doi.org/10.1515/BC.1999.050
Dashti NH, Abidin RS, Sains-bury F (2018) Programmable coencapsidation of guest proteins for intracellular delivery by virus-like particles. ACS Nano 12(5):4615–4623
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Dashti, N.H., Sainsbury, F. (2020). Virus-Derived Nanoparticles. In: Gerrard, J., Domigan, L. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 2073. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9869-2_9
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DOI: https://doi.org/10.1007/978-1-4939-9869-2_9
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