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Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging

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Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2069))

Abstract

In vivo whole-animal optical (bioluminescence and fluorescence) imaging of Staphylococcus aureus infections has provided the opportunity to noninvasively and longitudinally monitor the dynamics of the bacterial burden and ensuing host immune responses in live anesthetized animals. Herein, we describe several different mouse models of S. aureus skin infection, skin inflammation, incisional/excisional wound infections, as well as mouse and rabbit models of orthopedic implant infection, which utilized this imaging technology. These animal models and imaging methodologies provide insights into the pathogenesis of these infections and innate and adaptive immune responses, as well as the preclinical evaluation of diagnostic and treatment modalities. Noninvasive approaches to investigate host-pathogen interactions are extremely important as virulent community-acquired methicillin-resistant S. aureus strains (CA-MRSA) are spreading through the normal human population, becoming more antibiotic resistant and creating a serious threat to public health.

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Acknowledgments

This work was supported by the National Institutes of Arthritis and Musculoskeletal and Skin Diseases (grant numbers: R01AR069502 and R01AR073665 [to L.S.M.]), and the National Institute of Allergy and Infectious Diseases (grant numbers: R21AI126896 [to L.S.M.] and R01 AI047294 [to S.S.] and R56 AI103687 [to S.I.S.]) from the US National Institutes of Health, Department of Health and Human Services. The content is solely the responsibility of the authors and does not necessarily represent the official views of the US National Institutes of Health.

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Authors and Affiliations

  1. Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Nathan K. Archer, Yu Wang, Roger V. Ortines, Haiyun Liu, Sabrina J. Nolan, Qi Liu, Martin P. Alphonse, Dustin A. Dikeman, Momina Mazhar, Robert J. Miller & Lloyd S. Miller

  2. Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA

    Leif S. Anderson & Scott I. Simon

  3. PerkinElmer, Inc., Hopkinton, MA, USA

    Kevin P. Francis

  4. Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Lloyd S. Miller

  5. Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Lloyd S. Miller

  6. Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD, USA

    Lloyd S. Miller

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  1. Nathan K. Archer
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  2. Yu Wang
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  14. Lloyd S. Miller
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Correspondence to Lloyd S. Miller .

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  1. Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA

    Yinduo Ji

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Archer, N.K. et al. (2020). Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging. In: Ji, Y. (eds) Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols. Methods in Molecular Biology, vol 2069. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9849-4_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9849-4_15

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