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Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols pp 177–186Cite as

Metabolomic Profiling of Staphylococcus aureus

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2069))

Abstract

Metabolomics is becoming increasingly important in bioscience research as it provides a comprehensive analytical platform for a better understanding of the metabolic functions of cells and organisms. Recently, microbial metabolomics has been utilized in diverse research areas, including detection and diagnosis of pathogens, metabolic engineering, and drug discovery. An efficient and reproducible method to measure the intracellular metabolites of a specific microbial organism is a key prerequisite for utilizing metabolome analysis in microbiological research. In this chapter, we describe a workflow focusing on the extraction and quantification of intracellular metabolites of Staphylococcus aureus. Fast quenching with chilled methanol is applied to minimize metabolite leakage, while solvent extraction is used to obtain both polar and nonpolar fractions, which are then analyzed by respective liquid chromatography-mass spectrometry (LC-MS) methods for characterizing and quantifying the intracellular metabolites of S. aureus. This protocol is demonstrated to be an efficient method for analyzing polar and nonpolar intracellular metabolites of S. aureus.

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Acknowledgments

This work was supported by grant AI057451 from the National Institute of Allergy and Infectious Disease and partially supported by grant MIN-63-082 from CVM research Office UMN Ag Experimental Station General Agricultural Research Funds.

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Authors and Affiliations

  1. Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St Paul, MN, USA

    Ting Lei & Yinduo Ji

  2. Department of Food Science and Nutrition, University of Minnesota, St Paul, MN, USA

    Qingqing Mao & Chi Chen

Authors
  1. Ting Lei
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  2. Qingqing Mao
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  3. Chi Chen
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  4. Yinduo Ji
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Corresponding author

Correspondence to Yinduo Ji .

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Editors and Affiliations

  1. Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA

    Yinduo Ji

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Lei, T., Mao, Q., Chen, C., Ji, Y. (2020). Metabolomic Profiling of Staphylococcus aureus. In: Ji, Y. (eds) Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols. Methods in Molecular Biology, vol 2069. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9849-4_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9849-4_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9848-7

  • Online ISBN: 978-1-4939-9849-4

  • eBook Packages: Springer Protocols

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