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Digital Holographic Imaging as a Method for Quantitative, Live Cell Imaging of Drug Response to Novel Targeted Cancer Therapies

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Theranostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2054))

Abstract

Digital holographic imaging (DHI) is a noninvasive, live cell imaging technique that enables long-term quantitative visualization of cells in culture. DHI uses phase-shift imaging to monitor and quantify cellular events such as cell division, cell death, cell migration, and drug responses. In recent years, the application of DHI has expanded from its use in the laboratory to the clinical setting, and currently it is being developed for use in theranostics. Here, we describe the use of the DHI platform HoloMonitorM4 to evaluate the effects of novel, targeted cancer therapies on cell viability and proliferation using the HeLa cancer cell line as a model. We present single cell tracking and population-wide analysis of multiple cell morphology parameters.

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Author information

Authors and Affiliations

  1. Faculty of Health, School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Cancer and Ageing Research Program, Translational Research Institute, Queensland University of Technology, Woolloongabba, QLD, Australia

    Laura V. Croft, Derek J. Richard & Kenneth O’Byrne

  2. TrendBio Pty Ltd., Alphington, VIC, Australia

    Jaimie A. Mulders

  3. Cancer Services, Princess Alexandra Hospital, Brisbane, QLD, Australia

    Kenneth O’Byrne

Authors
  1. Laura V. Croft
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  2. Jaimie A. Mulders
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  3. Derek J. Richard
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  4. Kenneth O’Byrne
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Corresponding author

Correspondence to Laura V. Croft .

Editor information

Editors and Affiliations

  1. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Jyotsna Batra

  2. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Srilakshmi Srinivasan

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Croft, L.V., Mulders, J.A., Richard, D.J., O’Byrne, K. (2019). Digital Holographic Imaging as a Method for Quantitative, Live Cell Imaging of Drug Response to Novel Targeted Cancer Therapies. In: Batra, J., Srinivasan, S. (eds) Theranostics. Methods in Molecular Biology, vol 2054. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9769-5_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9769-5_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9768-8

  • Online ISBN: 978-1-4939-9769-5

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