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Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii

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Cryptosporidium

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2052))

Abstract

Development of an effective vaccine against cryptosporidiosis is a medical and veterinary priority. However, many putative Cryptosporidium vaccine candidates such as surface and apical complex antigens are posttranslationally modified with O- and N-linked glycans. This presents a significant challenge to understanding the functions of these antigens and the immune responses to them. Isolation of large amounts of native antigen from Cryptosporidium oocysts is expensive and is only feasible for C. parvum antigens. Here, we describe a method of producing recombinant, functional Cryptosporidium glycoprotein antigens in Toxoplasma gondii. These functional recombinant proteins can be used to investigate the role of glycotopes in Cryptosporidium immune responses and parasite–host cell interactions.

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Author information

Authors and Affiliations

  1. Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, USA

    Iwona Driskell & Roberta M. O’Connor

Authors
  1. Iwona Driskell
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  2. Roberta M. O’Connor
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Corresponding author

Correspondence to Roberta M. O’Connor .

Editor information

Editors and Affiliations

  1. Atlanta Veterans Affairs Medical Center, Decatur, GA, USA

    Jan R. Mead

  2. Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA

    Michael J. Arrowood

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Driskell, I., O’Connor, R.M. (2020). Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii. In: Mead, J., Arrowood, M. (eds) Cryptosporidium. Methods in Molecular Biology, vol 2052. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9748-0_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9748-0_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9747-3

  • Online ISBN: 978-1-4939-9748-0

  • eBook Packages: Springer Protocols

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