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3D Nanochannel Electroporation for Macromolecular Nucleotide Delivery

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Electroporation Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2050))

Abstract

Delivery of macromolecular nucleotides into the living cells holds a great promise for the development of new therapeutics. However, its abilities for adoptive immunotherapy, cell reprogramming, and primary cell transfection have been long-term hindered by the lack of a system that can locally deliver engineered therapeutic nucleotides (e.g., plasmids, siRNAs, miRNAs) without causing any side effects. In this chapter, the performance of a novel 3D nanoelectroporation system (3D NEP) is highlighted in three scenarios—adoptive immunotherapy, cell reprogramming, and adult mouse primary cardiomyocyte transfection. Detailed protocols were given to introduce the 3D NEP system assembly, as well as their applications in (1) natural killer (NK) cells transfection by delivery of chimeric antigen receptor (CAR) plasmids; (2) mouse embryonic fibroblasts transfection with OSKM factors; and (3) miR-29b molecular beacon (BMs) delivery into primary cardiomyocytes for interrogating the side effect of miR-29b-assisted treatment.

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Author information

Authors and Affiliations

  1. School of Biological Science and Medical Engineering, Beihang University, Beijing, China

    Lingqian Chang

  2. Institute of Nanotechnology for Single Cell Analysis (INSCA), Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing, China

    Lingqian Chang

  3. Department of Biomedical Engineering, University of North Texas, Denton, TX, USA

    Chandani Chitrakar & Mehdi Nouri

Authors
  1. Lingqian Chang
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  2. Chandani Chitrakar
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  3. Mehdi Nouri
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Corresponding author

Correspondence to Lingqian Chang .

Editor information

Editors and Affiliations

  1. MD Anderson Cancer Center, The University of Texas, Houston, TX, USA

    Shulin Li

  2. School of Biological Science and Medical Engineering, Beihang University, Beijing, China, Institute of Nanotechnology for Single Cell Analysis (INSCA), Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University,, Beijing, China

    Lingqian Chang

  3. Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France

    Justin Teissie

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Chang, L., Chitrakar, C., Nouri, M. (2020). 3D Nanochannel Electroporation for Macromolecular Nucleotide Delivery. In: Li, S., Chang, L., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 2050. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9740-4_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9740-4_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9739-8

  • Online ISBN: 978-1-4939-9740-4

  • eBook Packages: Springer Protocols

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