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Two-Photon Glutamate Uncaging to Study Structural and Functional Plasticity of Dendritic Spines

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Multiphoton Microscopy

Part of the book series: Neuromethods ((NM,volume 148))

Abstract

The activity-dependent structural remodeling of dendritic spines in response to sensory experience is vital for the dynamic regulation of neuronal circuit connectivity that supports complex behavior. Here, we discuss how the two-photon glutamate uncaging technique can be applied to study the mechanisms that drive activity-dependent structural and functional plasticity in individual dendritic spines. Our goal is to provide the reader with the key background for this technique and to present guidelines, practical details, and potential caveats associated with its implementation.

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Acknowledgments

This research was supported by NIH grants R01 NS062736 and U01 NS103571 from the National Institute of Neurological Disorders and Stroke.

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Authors and Affiliations

  1. Center for Neuroscience, University of California, Davis, CA, USA

    Ivar S. Stein & Karen Zito

  2. Department of Neurosurgery, New York University, New York, NY, USA

    Travis C. Hill

  3. Department of Pharmacology, University of Colorado Anschutz Medical Campus, Denver, CO, USA

    Won Chan Oh

  4. Department of Cell Biology and Neuroscience, Juntendo University, Tokyo, Japan

    Laxmi K. Parajuli

Authors
  1. Ivar S. Stein
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  2. Travis C. Hill
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  3. Won Chan Oh
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  4. Laxmi K. Parajuli
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  5. Karen Zito
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Corresponding author

Correspondence to Karen Zito .

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Editors and Affiliations

  1. Department of Biomedicine, University of Bergen, Bergen, Norway

    Espen Hartveit

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Stein, I.S., Hill, T.C., Oh, W.C., Parajuli, L.K., Zito, K. (2019). Two-Photon Glutamate Uncaging to Study Structural and Functional Plasticity of Dendritic Spines. In: Hartveit, E. (eds) Multiphoton Microscopy. Neuromethods, vol 148. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9702-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9702-2_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9701-5

  • Online ISBN: 978-1-4939-9702-2

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