Abstract
Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are among the most prevalent causes of sexually transmitted infections (STIs) worldwide. Timely and accurate diagnosis plays an important role in deciding appropriate treatment and preventing the spread of the infection. Strand invasion based amplification (SIBA), is an established isothermal nucleic acid amplification method for the rapid and accurate detection of infectious diseases. SIBA was applied for the simultaneous detection of CT and NG in less than 1 h. The multiplex SIBA (mSIBA) method displayed high analytical sensitivity and specificity for the detection of CT and NG. Since the method is performed at low and constant temperature, it can therefore be run on portable instruments. SIBA enables rapid screening for CT and NG within point-of-care or central laboratory settings.
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References
Centers for Disease Control and Prevention (2014) Sexually transmitted disease surveillance 2013. U.S. Department of Health and Human Services, Atlanta, p 2014
Baugh LR, Hill AA, Brown EL, Hunter CP (2001) Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29(5):e29. https://doi.org/10.1093/nar/29.5.e29
Newman L, Rowley J, Vander Hoorn S, Wijesooriya NS, Unemo M, Low N, Stevens G, Gottlieb S, Kiarie J, Temmerman M (2015) Global estimates of the prevalence and incidence of four curable sexually transmitted infections in 2012 based on systematic review and global reporting. PLoS One 10(12):e0143304. https://doi.org/10.1371/journal.pone.0143304
Papp JR, Schachter J, Gaydos CA, Van Der Pol B (2014) Recommendations for the laboratory-based detection of Chlamydia trachomatis and Neisseria gonorrhoeae — 2014. MMWR Recomm Rep 63(RR-02):1–19
Van Dyck E, Ieven M, Pattyn S, Van Damme L, Laga M (2001) Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by enzyme immunoassay, culture, and three nucleic acid amplification tests. J Clin Microbiol 39(5):1751–1756. https://doi.org/10.1128/jcm.39.5.1751-1756.2001
Gaydos CA, Quinn TC, Willis D, Weissfeld A, Hook EW, Martin DH, Ferrero DV, Schachter J (2003) Performance of the APTIMA combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens. J Clin Microbiol 41(1):304–309. https://doi.org/10.1128/jcm.41.1.304-309.2003
Hoser MJ, Mansukoski HK, Morrical SW, Eboigbodin KE (2014) Strand invasion based amplification (SIBA®): a novel isothermal DNA amplification technology demonstrating high specificity and sensitivity for a single molecule of target analyte. PLoS One 9(11):e112656. https://doi.org/10.1371/journal.pone.0112656
Eboigbodin KE, Hoser MJ (2016) Multiplex strand invasion based amplification (mSIBA) assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. Sci Rep 6:20487. https://doi.org/10.1038/srep20487
Eboigbodin KE, Moilanen K, Elf S, Hoser M (2017) Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA® BMC Infectious Diseases 17(1):134. https://doi.org/10.1186/s12879-017-2227-x
Eboigbodin KE, Brummer M, Ojalehto T, Hoser M (2016) Rapid molecular diagnostic test for Zika virus with low demands on sample preparation and instrumentation. Diagn Microbiol Infect Dis 86(4):369–371. https://doi.org/10.1016/j.diagmicrobio.2016.08.027
Eboigbodin K, Filén S, Ojalehto T, Brummer M, Elf S, Pousi K, Hoser M (2016) Reverse transcription strand invasion based amplification (RT-SIBA): a method for rapid detection of influenza A and B. Appl Microbiol Biotechnol:1–9. https://doi.org/10.1007/s00253-016-7491-y
Formosa T, Alberts BM (1986) Purification and characterization of the T4 bacteriophage uvsX protein. J Biol Chem 261(13):6107–6118
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Eboigbodin, K.E., Hoser, M. (2019). Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion Based Amplification (mSIBA). In: Brown, A. (eds) Chlamydia trachomatis. Methods in Molecular Biology, vol 2042. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9694-0_1
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DOI: https://doi.org/10.1007/978-1-4939-9694-0_1
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