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NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds

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G-Quadruplex Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2035))

Abstract

G-quadruplexes are noncanonical, four-stranded nucleic acid secondary structures formed in sequences containing consecutive runs of guanines. These G-quadruplex structures have been found to form in nucleic acid regions of biological significance, including human telomeres, gene promoters, and untranslated regions of mRNA. Thus, they are considered attractive therapeutic targets. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method for understanding the structures of G-quadruplexes and their interactions with small molecules under physiologically relevant conditions. Here, we present the NMR methodology used in our research group for the study of DNA G-quadruplex structures in physiologically relevant solution and their ligand interactions.

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Acknowledgments

This research was supported by the National Institutes of Health (R01CA122952 (DY), R01CA177585 (DY), and P30CA023168 (Purdue Center for Cancer Research)).

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Authors and Affiliations

  1. Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA

    Clement Lin & Jonathan Dickerhoff

  2. Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA

    Danzhou Yang

  3. Purdue Center for Cancer Research, West Lafayette, IN, USA

    Danzhou Yang

  4. Purdue Institute for Drug Discovery, West Lafayette, IN, USA

    Danzhou Yang

Authors
  1. Clement Lin
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  2. Jonathan Dickerhoff
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  3. Danzhou Yang
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Corresponding author

Correspondence to Danzhou Yang .

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Editors and Affiliations

  1. Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, Purdue Center for Cancer Research, Purdue Institute for Drug Discovery, West Lafayette, IN, USA

    Danzhou Yang

  2. Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA

    Clement Lin

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Lin, C., Dickerhoff, J., Yang, D. (2019). NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds. In: Yang, D., Lin, C. (eds) G-Quadruplex Nucleic Acids. Methods in Molecular Biology, vol 2035. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9666-7_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9666-7_9

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9665-0

  • Online ISBN: 978-1-4939-9666-7

  • eBook Packages: Springer Protocols

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