Abstract
The delivery of nucleic acid to eukaryotic cells is challenging due to presence of various intra- and extracellular barriers and need to delivery carriers. However, current gene delivery carriers, including PLL, PEI, and liposome, suffer from nanocarrier associate toxicity, low efficiency and polydispersity, and non-biodegradability. Here we describe our strategy for developing safe, efficacious, and monodisperse peptide-based carrier for gene delivery. We explain the use of genetic engineering technology for integrating various functional motifs in a single peptide, with less than 100 nm size, which facilitated gene delivery into mammalian cell.
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Acknowledgments
The authors would like to thank Elaheh Emamgholizadeh, Fatemeh Rabbani, Behenam Hajipour, Farzad Yousefi, Roya Cheraghi, and Asia Majidi, for help in developing the various methods for characterization of peptide-based nanocarriers.
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Alipour, M., Hosseinkhani, S. (2019). Design, Preparation, and Characterization of Peptide-Based Nanocarrier for Gene Delivery. In: Weissig, V., Elbayoumi, T. (eds) Pharmaceutical Nanotechnology. Methods in Molecular Biology, vol 2000. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9516-5_5
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DOI: https://doi.org/10.1007/978-1-4939-9516-5_5
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-4939-9516-5
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