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Quantification of Double-Strand Breaks in Mammalian Cells Using Pulsed-Field Gel Electrophoresis

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DNA Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1999))

Abstract

The double-strand break (DSB) is the most cytotoxic type of DNA damage and measurement of DSBs in cells is essential to understand their induction and repair. Pulsed-field gel electrophoresis (PFGE) allows for quantitative measurement of DSBs in a cell population generated by DNA damaging agents. PFGE has the capacity to separate DNA molecules from several hundred base pairs to over six million base pairs. In the method described here, molecules from five hundred thousand to three million base pairs are consolidated into a single band on the gel that is readily analyzed.

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Author information

Authors and Affiliations

  1. Department of Cellular and Molecular Medicine, University of Arizona Cancer Center, University of Arizona, Tuscon, AZ, USA

    Kelvin W. Pond & Nathan A. Ellis

Authors
  1. Kelvin W. Pond
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  2. Nathan A. Ellis
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Corresponding author

Correspondence to Nathan A. Ellis .

Editor information

Editors and Affiliations

  1. Indiana University – Purdue University, Indianapolis, IN, USA

    Lata Balakrishnan

  2. Department of Biological Sciences, University of South Carolina, Columbia, SC, USA

    Jason A. Stewart

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Pond, K.W., Ellis, N.A. (2019). Quantification of Double-Strand Breaks in Mammalian Cells Using Pulsed-Field Gel Electrophoresis. In: Balakrishnan, L., Stewart, J. (eds) DNA Repair. Methods in Molecular Biology, vol 1999. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9500-4_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9500-4_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9499-1

  • Online ISBN: 978-1-4939-9500-4

  • eBook Packages: Springer Protocols

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