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Analysis of Host Responses to Neisseria gonorrhoeae Using a Human Three-Dimensional Endometrial Epithelial Cell Model

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Neisseria gonorrhoeae

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1997))

Abstract

Neisseria gonorrhoeae infections have been associated with complications including chronic endometritis and pelvic inflammatory disease. Robust in vitro models of the female reproductive tract are urgently needed to better understand the biological mechanisms leading to these pathophysiological changes. Our human three-dimensional (3D) endometrial epithelial cell (EEC) model, which is generated using the HEC-1A cell line and rotating wall vessel (RWV) bioreactor technology, replicates several hallmarks of endometrial tissue in vivo. Studying the interactions of N. gonorrhoeae with the host using this newly characterized human 3D EEC model allows for the investigation of unique mechanisms of gonococcal pathogenesis in the upper female reproductive tract. In this chapter, we describe methodologies that can be used to investigate the interactions of N. gonorrhoeae with the human 3D endometrial epithelium. Protocols for generating the human 3D EEC model using the RWV technology and assessing the host response (including morphological/ultrastructural changes to the epithelial cells; cytokine/chemokine secretion or gene expression changes) following infection with N. gonorrhoeae are presented.

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Acknowledgments

The authors would like to thank Dr. Magdalene So (Department of Immunobiology and BIO5 Institute, University of Arizona, Tucson, AZ) for providing N. gonorrhoeae strains, and Adriana Tonachio, and David Lowry (Electron Microscope Laboratory, Arizona State University, Tempe, AZ) for technical support with electron microscope analyses.

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Authors and Affiliations

  1. Department of Basic Medical Sciences, The University of Arizona College of Medicine-Phoenix, Phoenix, AZ, USA

    Paweł Łaniewski & Melissa M. Herbst-Kralovetz

  2. Department of Obstetrics and Gynecology, The University of Arizona College of Medicine-Phoenix, Phoenix, AZ, USA

    Melissa M. Herbst-Kralovetz

Authors
  1. Paweł Łaniewski
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  2. Melissa M. Herbst-Kralovetz
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Corresponding author

Correspondence to Melissa M. Herbst-Kralovetz .

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Editors and Affiliations

  1. Molecular Microbiology Group, Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK

    Myron Christodoulides

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Łaniewski, P., Herbst-Kralovetz, M.M. (2019). Analysis of Host Responses to Neisseria gonorrhoeae Using a Human Three-Dimensional Endometrial Epithelial Cell Model. In: Christodoulides, M. (eds) Neisseria gonorrhoeae. Methods in Molecular Biology, vol 1997. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9496-0_20

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  • DOI: https://doi.org/10.1007/978-1-4939-9496-0_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9495-3

  • Online ISBN: 978-1-4939-9496-0

  • eBook Packages: Springer Protocols

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