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Rhodococcus and Yarrowia-Based Lipid Production Using Lignin-Containing Industrial Residues

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Microbial Lipid Production

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1995))

Abstract

Improvement in biorefining technologies coupled with development of novel fermentation strategies and analysis will be paramount in establishing supplementary and sustainable biofuel pathways. Oleaginous microorganisms that are capable of accumulating triacylglycerides (TAGs) and fatty acid methyl esters (FAMEs), such as Rhodococcus and Yarrowia species, can be used to produce second-generation biofuels from non-food competing carbon sources. These “microbiorefineries” provide a pathway to upgrade agricultural and industrial waste streams to fungible fuels or precursors to chemicals and materials. Here we provide a general overview on cultivating Rhodococcus and Yarrowia on agro-waste/industrial biomass pretreatment waste streams to produce single-cell oils/lipids and preparing samples for FAME detection.

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Acknowledgments

This work was supported by US Department of Energy (award #DE—EE0006112), and we would like to acknowledge our collaborator Joshua S. Yuan at Texas A&M University.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, USA

    Rosemary K. Le, Kristina M. Mahan & Arthur J. Ragauskas

  2. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA

    Arthur J. Ragauskas

  3. Department of Forestry, Wildlife and Fisheries, Center of Renewable Carbon, Institute of Agriculture, University of Tennessee, Knoxville, TN, USA

    Arthur J. Ragauskas

Authors
  1. Rosemary K. Le
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  2. Kristina M. Mahan
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  3. Arthur J. Ragauskas
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Corresponding author

Correspondence to Arthur J. Ragauskas .

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Editors and Affiliations

  1. Department of Engineering Technology, Biotechnology Program College of Technology, University of Houston, Houston, TX, USA

    Venkatesh Balan

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Le, R.K., Mahan, K.M., Ragauskas, A.J. (2019). Rhodococcus and Yarrowia-Based Lipid Production Using Lignin-Containing Industrial Residues. In: Balan, V. (eds) Microbial Lipid Production. Methods in Molecular Biology, vol 1995. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9484-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9484-7_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9483-0

  • Online ISBN: 978-1-4939-9484-7

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