Abstract
Microfluidic devices provide convenient assays tools for testing cell cultures in three-dimensional (3D) formats. These devices have significant potential for establishing assays that are better at predicting drug toxicity and efficacy effects compared to assays in conventional two-dimensional (2D) cultures. Microfluidic cell culture devices consist of perfused cell culture chambers with inlets and outlet for seeding, culturing, sampling, and assaying the cells. This protocol describes how to prepare and seed cells in a microfluidic cell culture device for drug toxicity testing on cells in a 3D structure. The protocol exemplifies the use of a basic microfluidic device with HepG2 hepatoma cells but can be transferred and optimized for other cells and cell types, including iPSC-derived tissue and organ cells.
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Acknowledgments
The research leading to these results has received support from the Innovative Medicines Initiative Joint Undertaking under (grant no. 115439), resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007-2013) and EFPIA companies. This publication reflects only the author’s views, and neither the IMI JU nor EFPIA nor the European Commission is liable for any use that may be made of the information contained therein.
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Christoffersson, J., Mandenius, CF. (2019). Using a Microfluidic Device for Culture and Drug Toxicity Testing of 3D Cells. In: Mandenius, CF., Ross, J. (eds) Cell-Based Assays Using iPSCs for Drug Development and Testing. Methods in Molecular Biology, vol 1994. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9477-9_22
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DOI: https://doi.org/10.1007/978-1-4939-9477-9_22
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-4939-9477-9
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