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Testing the Impact of Protease Inhibitors in Antigen Presentation Assays

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Antigen Processing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1988))

Abstract

The major histocompatibility complex (MHC) class I restricted pathway of antigen processing allows the presentation of intracellular antigens to cytotoxic T lymphocytes. The proteasome is the main protease in the cytoplasm and the nucleus, which is responsible for the generation of most peptide ligands of MHC-I molecules. Peptides produced by the proteasome can be further trimmed or destroyed by numerous cytosolic or endoplasmic reticulum (ER) luminal proteases. Small molecule inhibitors are useful tools for probing the role of proteases in MHC class I antigen processing. Here, we describe different methods to test the impact of protease inhibitors in antigen presentation assays.

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Acknowledgments

This work was supported by grants from the German Research Foundation grant Nr. BA 4199/2-1 to M.B. and GR 1517/2.4 and GR 1517/10-2 to M.G., the SwissLife Jubiläumsstiftung to M.B., and Swiss Cancer Research grant KFS-3687-08-2015 to M.G.

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Authors and Affiliations

  1. Biotechnology Institute Thurgau at the University of Konstanz, Kreuzlingen, Switzerland

    Michael Basler & Marcus Groettrup

  2. Division of Immunology, Department of Biology, University of Konstanz, Konstanz, Germany

    Michael Basler & Marcus Groettrup

Authors
  1. Michael Basler
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  2. Marcus Groettrup
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Corresponding author

Correspondence to Michael Basler .

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Editors and Affiliations

  1. INSERM U1151, Université Paris Descartes, Hopital Necker-Enfants Malades, Paris, France

    Peter van Endert

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Basler, M., Groettrup, M. (2019). Testing the Impact of Protease Inhibitors in Antigen Presentation Assays. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology, vol 1988. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9450-2_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9450-2_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9449-6

  • Online ISBN: 978-1-4939-9450-2

  • eBook Packages: Springer Protocols

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