Abstract
Single-cell technologies have emerged as advanced tools to study various biological processes that demand the single cell resolution. To detect subtle heterogeneity in the transcriptome, high accuracy and sensitivity are still desired for single-cell RNA-seq. We describe here multiple annealing and dC-tailing-based quantitative single-cell RNA-seq (MATQ-seq) with ~90% capture efficiency. In addition, MATQ-seq is a total RNA assay allowing for detection of nonpolyadenylated transcripts.
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Acknowledgments
This work is supported by McNair Scholarship and McNair Single Cell Initiative. We are grateful to McNair family and Dr. Neblett for their kindly support. We would like to thank Wenjian Cao, Yichi Niu, Dr. Zhiying Hu, and Dr. Yanhua Zhao for their contribution to the development of MATQ-seq.
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Sheng, K., Zong, C. (2019). Single-Cell RNA-Seq by Multiple Annealing and Tailing-Based Quantitative Single-Cell RNA-Seq (MATQ-Seq). In: Proserpio, V. (eds) Single Cell Methods. Methods in Molecular Biology, vol 1979. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9240-9_5
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DOI: https://doi.org/10.1007/978-1-4939-9240-9_5
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