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Examining Gene Expression Patterns Through Whole-Mount In Situ Hybridization

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Developmental Toxicology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1965))

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Abstract

RNA in situ hybridization is a practical technique that allows investigators to observe temporal and spatial gene expression at the RNA level in the context of whole embryos or tissues. One powerful application of in situ hybridization is to observe the consequences of genetic, toxicologic, or environmental perturbations on gene expression or morphogenesis during development. Herein, I will review the procedure to perform nonradioactive, in situ hybridization on whole-mount mouse or chick embryos.

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Author information

Authors and Affiliations

  1. Department of Physiology and Developmental Biology, College of Life Sciences, Brigham Young University, Provo, UT, USA

    Jeffery R. Barrow

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  1. Jeffery R. Barrow
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Correspondence to Jeffery R. Barrow .

Editor information

Editors and Affiliations

  1. Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA

    Jason M. Hansen

  2. Biomedical and Molecular Sciences, Queen’s University, Kingston, ON, Canada

    Louise M. Winn

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Barrow, J.R. (2019). Examining Gene Expression Patterns Through Whole-Mount In Situ Hybridization. In: Hansen, J., Winn, L. (eds) Developmental Toxicology. Methods in Molecular Biology, vol 1965. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9182-2_19

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  • DOI: https://doi.org/10.1007/978-1-4939-9182-2_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9181-5

  • Online ISBN: 978-1-4939-9182-2

  • eBook Packages: Springer Protocols

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