Abstract
Lymphocytes are an important component of inflammatory bowel disease (IBD) pathogenesis. T cells in particular are potent inducers of inflammation in this disease as well as recurrent players in chronic lesion formation. Here we describe a method for adoptive transfer colitis in mice, which serves as a T cell-based model of inflammatory bowel disease. Adoptive transfer utilizes naïve CD4+ T cells that are administered to immunodeficient mice, which then induce a chronic pancolitis. Here, protocols are provided for the isolation and purification of naïve CD4+ T cells, as well as proper administration to test mice and important points to consider in monitoring disease progression and potential downstream applications. This method provides a way to target the contribution of T cells to IBD models, as well as providing a repeatable and physiologically relevant model of disease.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Dahlhamer JM, Zammitti EP, Ward BW et al (2016) Prevalence of inflammatory bowel disease among adults aged >/=18 years - United States, 2015. MMWR Morb Mortal Wkly Rep 65(42):1166–1169
Strober W, Fuss I, Mannon P (2007) The fundamental basis of inflammatory bowel disease. J Clin Invest 117(3):514–521
Elson CO, Cong Y, McCracken VJ et al (2005) Experimental models of inflammatory bowel disease reveal innate, adaptive, and regulatory mechanisms of host dialogue with the microbiota. Immunol Rev 206:260–276
Eri R, McGuckin MA, Wadley R (2012) T cell transfer model of colitis: a great tool to assess the contribution of T cells in chronic intestinal inflammation. Methods Mol Biol 844:261–275
Steinbach EC, Gipson GR, Sheikh SZ (2015) Induction of murine intestinal inflammation by adoptive transfer of effector CD4+ CD45RB high T cells into immunodeficient mice. J Vis Exp (98)
Ostanin DV, Bao J, Koboziev I et al (2009) T cell transfer model of chronic colitis: concepts, considerations, and tricks of the trade. Am J Physiol Gastrointest Liver Physiol 296(2):G135–G146
Kiesler P, Fuss IJ, Strober W (2015) Experimental models of inflammatory bowel diseases. Cell Mol Gastroenterol Hepatol 1(2):154–170
Feng T, Wang L, Schoeb TR et al (2010) Microbiota innate stimulation is a prerequisite for T cell spontaneous proliferation and induction of experimental colitis. J Exp Med 207(6):1321–1332
Kieper WC, Troy A, Burghardt JT et al (2005) Recent immune status determines the source of antigens that drive homeostatic T cell expansion. J Immunol 174(6):3158–3163
Ostanin DV, Pavlick KP, Bharwani S et al (2006) T cell-induced inflammation of the small and large intestine in immunodeficient mice. Am J Physiol Gastrointest Liver Physiol 290(1):G109–G119
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Eden, K. (2019). Adoptive Transfer Colitis. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1960. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9167-9_18
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9167-9_18
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9166-2
Online ISBN: 978-1-4939-9167-9
eBook Packages: Springer Protocols