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Lymphoma pp 77–103Cite as

PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1956))

Abstract

Assessment of the presence of clonal lymphoproliferations via polymerase chain reaction (PCR)-based analysis of rearranged immunoglobulin (IG) or T-cell receptor (TR) genes is a valuable method in the diagnosis of suspect lymphoproliferative disorders. Additionally, this methodology can be used for evaluating dissemination of lymphoma cells and for studying the clonal relationship between multiple (different locations) or consecutive (over time) lymphomas. Here we describe an integrated approach to assess clonality via analysis of Ig heavy chain (IGH), Ig kappa (IGK), TCR beta (TRB), and TCR gamma (TRG) gene rearrangements, based on the standardized multiplex PCRs as originally developed by the European BIOMED-2 consortium. The described protocol covers the pre-analytical phase of DNA isolation (from formalin-fixed paraffin-embedded and fresh tissues, body fluids, peripheral blood, and bone marrow), the analytical phase of PCR GeneScan and heteroduplex analysis, and the post-analytical interpretation of the obtained profiles, following established guidelines.

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Author information

Authors and Affiliations

  1. Laboratory for Molecular Diagnostics, Department of Laboratory Medicine, AZ Delta Hospital, Roeselare, Belgium

    Elke Boone

  2. Laboratory Medical Immunology, Department of Immunology, Erasmus MC, Rotterdam, Netherlands

    Kim C. Heezen & Anton W. Langerak

  3. Department of Pathology, Radboud University Medical Center, Nijmegen, Netherlands

    Patricia J. T. A. Groenen

Authors
  1. Elke Boone
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  2. Kim C. Heezen
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  3. Patricia J. T. A. Groenen
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  4. Anton W. Langerak
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Consortia

On behalf of the EuroClonality Consortium

Corresponding author

Correspondence to Anton W. Langerak .

Editor information

Editors and Affiliations

  1. Institute of Cell Biology (Cancer Research), Medical School, University of Duisburg-Essen, Nordrhein-Westfalen, Essen, Germany

    Ralf Küppers

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Boone, E., Heezen, K.C., Groenen, P.J.T.A., Langerak, A.W., On behalf of the EuroClonality Consortium. (2019). PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations. In: Küppers, R. (eds) Lymphoma. Methods in Molecular Biology, vol 1956. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9151-8_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9151-8_4

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9150-1

  • Online ISBN: 978-1-4939-9151-8

  • eBook Packages: Springer Protocols

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