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Intraspinal and Intracortical Delivery of AAV Vectors for Intersectional Circuit Tracing in Non-transgenic Species

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Adeno-Associated Virus Vectors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1950))

Abstract

The mapping of long-range axonal projections is a rapidly growing endeavor in the field of neuroscience. Recent advances in the development of adeno-associated viral vectors that can achieve efficient retrograde transport now enable the characterization and manipulation of specific neuronal subpopulations using Cre-dependent, intersectional approaches. Importantly, these approaches can be applied to non-transgenic animals, making it possible to carry out detailed anatomical studies across a variety of species including nonhuman primates. In this chapter, we demonstrate the utility of such intersectional strategies by describing methods for targeting viral constructs to distinct subsets of corticospinal motor neurons based on their projections to specific spinal cord segments.

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Acknowledgments

This work was supported by the Craig H. Neilsen Foundation, Paralyzed Veterans of America Research Foundation, and TIRR Foundation (J.N.D.).

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Authors and Affiliations

  1. Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA

    James M. Conner

  2. Department of Biology, Texas A&M University, College Station, TX, USA

    Greg L. Bain & Jennifer N. Dulin

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  1. James M. Conner
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  2. Greg L. Bain
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  3. Jennifer N. Dulin
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Correspondence to Jennifer N. Dulin .

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Editors and Affiliations

  1. Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA

    Michael J. Castle

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Conner, J.M., Bain, G.L., Dulin, J.N. (2019). Intraspinal and Intracortical Delivery of AAV Vectors for Intersectional Circuit Tracing in Non-transgenic Species. In: Castle, M. (eds) Adeno-Associated Virus Vectors. Methods in Molecular Biology, vol 1950. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9139-6_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9139-6_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9138-9

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