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Determination of Cell-Surface Hyaluronan Through Flow Cytometry

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The Extracellular Matrix

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1952))

Abstract

Hyaluronan is the major glycosaminoglycan present in the extracellular matrix of several cell types; its synthesis occurs on the cellular plasma membrane. Variations in its expression are related to alterations in cell proliferation, adhesion, and migration. It is able to interact with different binding proteins called hyaladherins, which can be conjugated to different fluorochromes and analyzed by flow cytometry.

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Author information

Authors and Affiliations

  1. Laboratorio de Microambiente Tumoral, Universidad Nacional del Noroeste de la Pcia. de Bs. As, Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA UNNOBA-CONICET), Junín, Buenos Aires, Argentina

    Daiana L. Vitale, Fiorella M. Spinelli & Laura Alaniz

Authors
  1. Daiana L. Vitale
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  2. Fiorella M. Spinelli
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  3. Laura Alaniz
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Corresponding author

Correspondence to Laura Alaniz .

Editor information

Editors and Affiliations

  1. Department of Medicine and Surgery, University of Insubria, Varese, Italy

    Davide Vigetti

  2. Department of Chemistry, University of Patras, Patras, Greece

    Achilleas D. Theocharis

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Vitale, D.L., Spinelli, F.M., Alaniz, L. (2019). Determination of Cell-Surface Hyaluronan Through Flow Cytometry. In: Vigetti, D., Theocharis, A.D. (eds) The Extracellular Matrix. Methods in Molecular Biology, vol 1952. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9133-4_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9133-4_10

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9132-7

  • Online ISBN: 978-1-4939-9133-4

  • eBook Packages: Springer Protocols

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