Abstract
Integrin-mediated interactions of cells with the extracellular matrix (ECM) are important for their activities. The ECM itself is a complex network of macromolecules forming aggregates or suprastructures. Moreover, the molecular composition is important for the macromolecular organization and, thereby, the functional properties of the ECM. In addition, collagen molecules lose their integrin-binding capabilities after incorporation into fibrils. Therefore, we have established detailed protocols for the analysis of integrin-matrix interactions at the supramolecular level.
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Acknowledgments
This work was supported by Deutsche Forschungsgemeinschaft grants BR1497/4-1 and EB177/9-1 to PB and JAE and a grant from BBSRC UK (BB/I011226/1) to BL.
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Hansen, U. (2019). Analysis of Collagen-Binding Integrin Interactions with Supramolecular Aggregates of the Extracellular Matrix. In: Sagi, I., Afratis, N. (eds) Collagen. Methods in Molecular Biology, vol 1944. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9095-5_12
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DOI: https://doi.org/10.1007/978-1-4939-9095-5_12
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