Abstract
Noninvasive, real-time optical imaging methods are well suited to follow the in vivo distribution of nucleic acid nanocarriers, their dissociation and the resulting gene expression or inhibition. Indeed, most small animal imaging devices are performing bioluminescence and fluorescence measurements without moving the animal, allowing a simple, rapid, and cost-effective method of investigation of several parameters at a time, in longitudinal experiments that can last for days or weeks.
Here we help the reader in choosing adapted near-infrared (NIR) fluorophores or pairs of fluorophores for FRET assays, imaging of reporter genes as well as nanocarriers for in vivo gene and siRNA delivery. In addition, we present the labeling methods of these macromolecules, and of their payload and the protocols to detect them using bioluminescence and NIR fluorescence imaging in mice.
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References
Weissleder R (2001) A clearer vision for in vivo imaging. Nat Biotechnol 19:316–317
Weissleder R, Ntziachristos V (2003) Shedding light onto live molecular targets. Nat Med 9:123–128
Josserand V, Texier-Nogues I, Huber P, Favrot MC, Coll JL (2007) Non-invasive in vivo optical imaging of the lacZ and luc gene expression in mice. Gene Ther 14:1587–1593
Lin EH, Keramidas M, Rome C, Chiu WT, Wu CW, Coll JL, Deng WP (2011) Lifelong reporter gene imaging in the lungs of mice following polyethyleneimine-mediated sleeping-beauty transposon delivery. Biomaterials 32:1978–1985
Morille M, Montier T, Legras P, Carmoy N, Brodin P, Pitard B, Benoit JP, Passirani C (2010) Long-circulating DNA lipid nanocapsules as new vector for passive tumor targeting. Biomaterials 31:321–329
Coll JL, Chollet P, Brambilla E, Desplanques D, Behr JP, Favrot M (1999) In vivo delivery to tumors of DNA complexed with linear polyethylenimine. Hum Gene Ther 10:1659–1666
Heurtault B, Saulnier P, Pech B, Proust JE, Benoit JP (2002) A novel phase inversion-based process for the preparation of lipid nanocarriers. Pharm Res 19:875–880
Morille M, Passirani C, Letrou-Bonneval E, Benoit JP, Pitard B (2009) Galactosylated DNA lipid nanocapsules for efficient hepatocyte targeting. Int J Pharm 379:293–300
Morille M, Passirani C, Dufort S, Bastiat G, Pitard B, Coll JL, Benoit JP (2011) Tumor transfection after systemic injection of DNA lipid nanocapsules. Biomaterials 32:2327–2333
Crombez L, Aldrian-Herrada G, Konate K, Nguyen QN, McMaster GK, Brasseur R, Heitz F, Divita G (2009) A new potent secondary amphipathic cell-penetrating peptide for siRNA delivery into mammalian cells. Mol Ther 17:95–103
Crombez L, Divita G (2011) A non-covalent peptide-based strategy for siRNA delivery. Methods Mol Biol 683:349–360
Acknowledgments
The authors thank C. Passirani, J.P. Benoit (INSERM Angers), I. Texier-Nogues (CEA-LETI, Grenoble), and P. Erbacher (Polyplus transfection, Illkirch, France) for their help and advices. This work was funded by the Agence Nationale pour la Recherche (ANR pNANO, CALIF, ANR BiotecS GLIOTHERAP, ANR CES NANOBIOTOX) and the INCA (PLbio Poro-Combo; PLBio Biosensimag).
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Rome, C. et al. (2019). Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo. In: Ogris, M., Sami, H. (eds) Nanotechnology for Nucleic Acid Delivery. Methods in Molecular Biology, vol 1943. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9092-4_23
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DOI: https://doi.org/10.1007/978-1-4939-9092-4_23
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