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Immunofluorescence Procedure for Developing Enamel Tissues

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Odontogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1922))

Abstract

Immunofluorescence (IF) labeling is a powerful technique that can provide a wealth of information on structural organization, supramolecular composition, and functional properties of cells and tissues. At the same time, nonspecific staining and false positives can seriously compromise IF studies and lead to confusing or even misleading results. It is particularly true for the extracellular matrix component of forming enamel. Here, we present an optimized IF protocol for developing enamel. Autofluorescence blocking by Sudan Black B (SBB) and establishing of proper isotype controls lead to a significant artifact reduction and improve reliability of the IF data.

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Author information

Authors and Affiliations

  1. Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USA

    Xu Yang & Elia Beniash

  2. Department of Bioengineering, Center for Craniofacial Regeneration, McGowan Institute for Regenerative Medicine, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA, USA

    Elia Beniash

Authors
  1. Xu Yang
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  2. Elia Beniash
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Corresponding author

Correspondence to Elia Beniash .

Editor information

Editors and Affiliations

  1. School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Petros Papagerakis

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Yang, X., Beniash, E. (2019). Immunofluorescence Procedure for Developing Enamel Tissues. In: Papagerakis, P. (eds) Odontogenesis. Methods in Molecular Biology, vol 1922. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9012-2_19

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  • DOI: https://doi.org/10.1007/978-1-4939-9012-2_19

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9011-5

  • Online ISBN: 978-1-4939-9012-2

  • eBook Packages: Springer Protocols

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