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Genetic Transformation of Cotton with the Harpin-Encoding Gene hpaXoo of Xanthomonas oryzae pv. oryzae and Evaluation of Resistance Against Verticillium Wilt

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Transgenic Cotton

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1902))

Abstract

The soilborne fungal pathogen Verticillium dahliae Kleb causes Verticillium wilt in a wide range of crops including cotton (Gossypium hirsutum). To date, most upland cotton varieties are susceptible to V. dahliae, and the breeding for cotton varieties with the resistance to Verticillium wilt has not been successful. Hpa1Xoo is a harpin protein from Xanthomonas oryzae pv. oryzae which induces the hypersensitive cell death in plants. When hpa1Xoo was transformed into the susceptible cotton line Z35 through Agrobacterium-mediated transformation, the transgenic cotton line (T-34) with an improved resistance to Verticillium dahliae was obtained. Here, we describe the related research approach, such as Western blot, Southern blot, immuno-gold labeling, evaluation of resistance to Verticillium dahliae, and how to detect the micro-hypersensitive response and oxidative burst elicited by harpinXoo in plant tissue.

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Authors and Affiliations

  1. College of Plant Protection, Hainan University, Haikou, People’s Republic of China

    Weiguo Miao

  2. College of Plant Protection, Nanjing Agricultural University, Nanjing, People’s Republic of China

    Jingsheng Wang

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  1. Weiguo Miao
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  2. Jingsheng Wang
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Correspondence to Weiguo Miao .

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Editors and Affiliations

  1. Department of Biology, East Carolina University, Greenville, NC, USA

    Baohong Zhang

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Miao, W., Wang, J. (2019). Genetic Transformation of Cotton with the Harpin-Encoding Gene hpaXoo of Xanthomonas oryzae pv. oryzae and Evaluation of Resistance Against Verticillium Wilt. In: Zhang, B. (eds) Transgenic Cotton. Methods in Molecular Biology, vol 1902. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8952-2_22

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  • DOI: https://doi.org/10.1007/978-1-4939-8952-2_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8951-5

  • Online ISBN: 978-1-4939-8952-2

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