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Recombinant Methioninase as a DNA Demethylation Agent

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Methionine Dependence of Cancer and Aging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1866))

Abstract

This chapter reviews the effect of methionine (MET) restriction, via treatment with recombinant methioninase (rMETase), on DNA methylation of cancer cells. CCRF-CEM human cancer cells were treated with rMETase under subcytotoxic conditions. The rMETase-treated cells contained significantly lower levels of genomic methylated DNA than did untreated control cells. DNA methylation was measured by incorporation of the methyl group of [3H]methyl-S-adenosylmethionine into DNA and by methylation-sensitive arbitrarily-primed PCR. DNA hypomethylation effected by rMETase was of similar extent to that effected by treatment of the cells with the DNA methyltransferase inhibitor 5-azacytidine.

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Author information

Authors and Affiliations

  1. AntiCancer, Inc., San Diego, CA, USA

    Robert M. Hoffman

  2. Department of Surgery, University of California, San Diego, CA, USA

    Robert M. Hoffman

  3. Hopital Paul Brousse, Villejuif Cedex, France

    David Machover

Authors
  1. Robert M. Hoffman
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  2. David Machover
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Corresponding author

Correspondence to Robert M. Hoffman .

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Editors and Affiliations

  1. AntiCancer, Inc., San Diego, CA, USA

    Robert M. Hoffman

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Hoffman, R.M., Machover, D. (2019). Recombinant Methioninase as a DNA Demethylation Agent. In: Hoffman, R. (eds) Methionine Dependence of Cancer and Aging. Methods in Molecular Biology, vol 1866. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8796-2_22

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  • DOI: https://doi.org/10.1007/978-1-4939-8796-2_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8795-5

  • Online ISBN: 978-1-4939-8796-2

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