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Continuous and Integrated Expression and Purification of Recombinant Antibodies

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Recombinant Protein Expression in Mammalian Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1850))

Abstract

This chapter introduces the necessary concepts to design continuous expression and purification processes for monoclonal antibodies. The operation of a perfusion bioreactor is discussed containing the preparation procedures, the seeding train and the preparation and control of a long-term production run. The downstream processes exploit the benefits of countercurrent chromatography. Their design from batch experiments is presented. The CaptureSMB process is introduced for continuous capturing while for polishing applications the design of the two-column MCSGP process is described. The chapter also puts these processes together in the context of their integration to an end-to-end production process.

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Acknowledgments

This work has been supported by the KTI (CTI)-Program of the Swiss Economic Ministry (Project 19190.2 PFIW-IW). The authors would like to thank Merck KGaA for supplying the Eshmuno A and CPX resin supporting this work. The authors declare that they have no conflicts of interest pertaining to the contents of this chapter.

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Author notes

  1. Sebastian Vogg and Moritz Kai Florian Wolf contributed equally to this chapter.

Authors and Affiliations

  1. ETH Zurich, Institute for Chemical and Bioengineering, Zurich, Switzerland

    Sebastian Vogg, Moritz Kai Florian Wolf & Massimo Morbidelli

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  1. Sebastian Vogg
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  2. Moritz Kai Florian Wolf
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  3. Massimo Morbidelli
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Correspondence to Massimo Morbidelli .

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Editors and Affiliations

  1. Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

    David L. Hacker

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Vogg, S., Wolf, M.K.F., Morbidelli, M. (2018). Continuous and Integrated Expression and Purification of Recombinant Antibodies. In: Hacker, D. (eds) Recombinant Protein Expression in Mammalian Cells. Methods in Molecular Biology, vol 1850. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8730-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-8730-6_11

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