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Imaging of Endothelial Cell Dynamic Behavior in Zebrafish

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Lymphangiogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1846))

Abstract

In recent years, use of the zebrafish embryo as a model organism to study vascular development in vivo has provided valuable insights into the genetic and cellular events shaping the embryonic vasculature. In this chapter, we aim to present the methods for the measurement of some of the most commonly investigated dynamic parameters in endothelial cells during developmental angiogenesis, namely, migration speed and acceleration, filopodia extension, front–rear polarity, cell cycle progression, membrane deformations, and junctional rearrangements. We also offer suggestions on how to deal with the most common imaging and quantifications challenges faced when acquiring and quantifying endothelial cell dynamic behavior in vivo.

We intend this section to serve as an experience-based imaging primer for scientists interested in endothelial cell imaging in the zebrafish embryo.

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Author information

Authors and Affiliations

  1. Integrative Vascular Biology Laboratory, Max-Delbrück Center for Molecular Medicine (MDC), Berlin, Germany

    Baptiste Coxam & Holger Gerhardt

  2. DZHK (German Center for Cardiovascular Research), Berlin, Germany

    Baptiste Coxam & Holger Gerhardt

  3. Berlin Institute of Health (BIH), Berlin, Germany

    Baptiste Coxam & Holger Gerhardt

  4. Vascular Patterning Laboratory, Vesalius Research Center, Leuven, Belgium

    Holger Gerhardt

  5. Department of Oncology, KU Leuven, Leuven, Belgium

    Holger Gerhardt

Authors
  1. Baptiste Coxam
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  2. Holger Gerhardt
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Corresponding author

Correspondence to Baptiste Coxam .

Editor information

Editors and Affiliations

  1. Center for Vascular & Developmental Biology, Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, IL, USA

    Guillermo Oliver

  2. Department of Medicine and Cardiovascular Institute, University of Pennsylvania, Philadelphia, PA, USA

    Mark L. Kahn

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Coxam, B., Gerhardt, H. (2018). Imaging of Endothelial Cell Dynamic Behavior in Zebrafish. In: Oliver, G., Kahn, M. (eds) Lymphangiogenesis. Methods in Molecular Biology, vol 1846. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8712-2_12

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  • DOI: https://doi.org/10.1007/978-1-4939-8712-2_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8711-5

  • Online ISBN: 978-1-4939-8712-2

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