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Natural Cotransformation and Multiplex Genome Editing by Natural Transformation (MuGENT) of Vibrio cholerae

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Vibrio Cholerae

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1839))

Abstract

Generating mutant strains is an essential component of microbial genetics. Natural genetic transformation, a process for the uptake and integration of foreign DNA, is shared by diverse microbial species and can be exploited for making mutant strains. Canonically, this process has been used to generate single mutants and sequentially to generate strains with multiple mutations. Recently, we have described a method for multiplex genome editing by natural transformation (MuGENT), which allows the generation of strains with multiple scarless mutations in a single step. Here, we provide a detailed description of the methods used for mutagenesis of the cholera pathogen Vibrio cholerae with a particular emphasis on mutagenesis via MuGENT.

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Authors and Affiliations

  1. Department of Biology, Indiana University, Bloomington, IN, USA

    Ankur B. Dalia

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  1. Ankur B. Dalia
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Correspondence to Ankur B. Dalia .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, Oregon, USA

    Aleksandra E. Sikora

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Dalia, A.B. (2018). Natural Cotransformation and Multiplex Genome Editing by Natural Transformation (MuGENT) of Vibrio cholerae . In: Sikora, A. (eds) Vibrio Cholerae. Methods in Molecular Biology, vol 1839. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8685-9_6

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  • DOI: https://doi.org/10.1007/978-1-4939-8685-9_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8684-2

  • Online ISBN: 978-1-4939-8685-9

  • eBook Packages: Springer Protocols

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