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Lipase-Catalyzed Synthesis of Structured Lipids at Laboratory Scale

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Lipases and Phospholipases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1835))

Abstract

In this chapter, some examples of laboratory protocols to produce functional structured lipids, namely, human milk fat substitutes, dietetic triacylglycerols, and interesterified fat blends with improved nutritional and rheological properties, catalyzed either by immobilized commercial or noncommercial lipase preparations, are presented. In addition to batch synthesis, the continuous production in packed- or fluidized-bed bioreactors is addressed, as well as the evaluation of operational stability of the biocatalysts used (either in batch reuses or in continuous mode).

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Acknowledgments

The authors acknowledge the national funding of FCT – Fundação para a Ciência e a Tecnologia, Portugal, to the research units of LEAF (UID/AGR/04129/2013) and MARE (UID/MAR/04292/2013).

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Authors and Affiliations

  1. Instituto Superior de Agronomia, Universidade de Lisboa, LEAF, Linking Landscape, Environment, Agriculture and Food, Lisbon, Portugal

    Suzana Ferreira-Dias & Carla Tecelão

  2. Escola Superior de Tecnologia do Barreiro, Instituto Politécnico de Setúbal, Barreiro, Portugal

    Natália M. Osório

  3. MARE—Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, Peniche, Portugal

    Carla Tecelão

Authors
  1. Suzana Ferreira-Dias
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  2. Natália M. Osório
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  3. Carla Tecelão
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Corresponding author

Correspondence to Suzana Ferreira-Dias .

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Editors and Affiliations

  1. CIATEJ, Guadalajara, Jalisco, Mexico

    Georgina Sandoval

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Ferreira-Dias, S., Osório, N.M., Tecelão, C. (2018). Lipase-Catalyzed Synthesis of Structured Lipids at Laboratory Scale. In: Sandoval, G. (eds) Lipases and Phospholipases. Methods in Molecular Biology, vol 1835. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8672-9_17

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  • DOI: https://doi.org/10.1007/978-1-4939-8672-9_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8671-2

  • Online ISBN: 978-1-4939-8672-9

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