Abstract
The visualization of biomolecules is a straightforward way to elucidate the physical properties of molecules and their reaction processes. Atomic force microscopy (AFM) enables the direct imaging of biomolecules under physiological conditions at nanometer-scale spatial resolution. Because AFM visualizes all molecules in a scanning area, an observation scaffold is required for the target-specific imaging of molecules in the dynamic state. The DNA origami technology allows the precise placement of target molecules in a designed nanostructure, and the detection of the molecules at the single-molecule level. DNA origami is applied for visualizing the detailed motions of molecules using high-speed AFM (HS-AFM), which enables the analysis of the dynamic movement of biomolecules in a subsecond time resolution. Here, we describe the combination of the DNA origami system with HS-AFM for the imaging of DNA structural changes controlled by photoresponsive molecules. The hybridization and dehybridization of photoresponsive oligonucleotides were visualized directly using this observation system. These target-oriented observation systems should contribute to the detailed analysis of biomolecules in real time with molecular resolution.
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Acknowledgments
This work was supported by JSPS KAKENHI (grant numbers 15H03837, 16K14033, 16H06356) and a Grant-in-Aid for Scientific Research on Innovative Areas “Molecular Robotics” (No. 24104002) from MEXT, Japan.
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Endo, M., Sugiyama, H. (2018). Direct Observation of Dynamic Movement of DNA Molecules in DNA Origami Imaged Using High-Speed AFM. In: Lyubchenko, Y. (eds) Nanoscale Imaging. Methods in Molecular Biology, vol 1814. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8591-3_13
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DOI: https://doi.org/10.1007/978-1-4939-8591-3_13
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