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Searching the GPCR Heterodimer Network (GPCR-hetnet) Database for Information to Deduce the Receptor–Receptor Interface and Its Role in the Integration of Receptor Heterodimer Functions

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Receptor-Receptor Interactions in the Central Nervous System

Part of the book series: Neuromethods ((NM,volume 140))

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Abstract

The G protein-coupled receptor heterocomplex network database (GPCR-hetnet) is a database designed to store information on GPCR heteroreceptor complexes and their allosteric receptor–receptor interactions. It is an expert-authored and peer-reviewed, curated collection of well-documented GPCR–GPCR interactions that span the gamut from classical GPCR–GPCR interactions to more complex receptor–receptor interactions (GPCR-Receptor Tyrosine Kinase and GPCR-ionotropic receptor/ligand gated ion channel). Although GPCR-hetnet contains interactions among GPCR from several different species, the curators have initially focused on receptor–receptor interactions in humans. Currently (August 2017) GPCR-hetnet contains information on 250 receptors (192 GPCR, 52 RTK, and 6 ionotropic receptors) and >1023 interactions. The GPCR-hetnet provides four searchable datasets: the hetnet, the non-hetnet, the rtknet, and the ionnet. Other supporting datasets include information about receptors that are present in GPCR-hetnet such as literature citations. This chapter describes in a basic protocol how to use, navigate, and browse through the GPCR-hetnet database to identify the clusters in which a receptor protomer of interest is involved, while further applicability are also described and introduced.

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Acknowledgments

The work was supported by the Swedish Medical Research Council (62X-00715-50-3) to K.F., by ParkinsonFonden 2015, AFA Försäkring (130328) to K.F., and by Hjärnfonden (FO2016-0302) and Karolinska Institutet Forskningsstiftelser (2016–2017) to D.O.B-E. D.O.B-E. belongs to the “Academia de Biólogos Cubanos” group.

Author information

Authors and Affiliations

  1. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    Ismel Brito & Dasiel O. Borroto-Escuela

  2. Observatorio Cubano de Neurociencias, Grupo Bohío-Estudio, Yaguajay, Cuba

    Ismel Brito & Dasiel O. Borroto-Escuela

  3. Facultad de Medicina, Instituto de Investigación Biomédica de Málaga, Universidad de Málaga, Málaga, Spain

    Manuel Narvaez

  4. Department of Biomolecular Science, Section of Physiology, University of Urbino, Urbino, Italy

    David Savelli, Michael Di Palma, Stefano Sartini, Riccardo Cuppini, Patrizia Ambrogini & Dasiel O. Borroto-Escuela

  5. Departamento de Biología Celular, Universidad de Málaga, Málaga, Spain

    Kirill Shumilov & Alicia Rivera

  6. Laboratory of Eukaryotic Gene Expression and Signal Transduction (LEGEST), Ghent University, Ghent, Belgium

    Kamila Skieterska & Kathleen Van Craenenbroeck

  7. The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, Stockholm, Sweden

    Ismael Valladolid-Acebes

  8. Departamento de Bioquímica, Facultat de Biología, Universidad de la Habana, La Habana, Cuba

    Rauner Zaldivar-Oro

  9. Laboratory of Drug Addiction Pharmacology, Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland

    Malgorzata Filip

  10. Campbell Research Institute, Centre for Addiction and Mental Health, University of Toronto, Toronto, ON, Canada

    Fang Liu

  11. Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico

    Miguel Pérez de la Mora

  12. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    Kjell Fuxe

Authors
  1. Ismel Brito
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  2. Manuel Narvaez
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  3. David Savelli
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  4. Kirill Shumilov
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  5. Michael Di Palma
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  6. Stefano Sartini
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  7. Kamila Skieterska
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  8. Kathleen Van Craenenbroeck
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  9. Ismael Valladolid-Acebes
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  10. Rauner Zaldivar-Oro
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  11. Malgorzata Filip
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  12. Riccardo Cuppini
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  13. Alicia Rivera
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  14. Fang Liu
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  15. Patrizia Ambrogini
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  16. Miguel Pérez de la Mora
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  17. Kjell Fuxe
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  18. Dasiel O. Borroto-Escuela
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Editor information

Editors and Affiliations

  1. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    KJELL FUXE

  2. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    Dasiel O. Borroto-Escuela

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Brito, I. et al. (2018). Searching the GPCR Heterodimer Network (GPCR-hetnet) Database for Information to Deduce the Receptor–Receptor Interface and Its Role in the Integration of Receptor Heterodimer Functions. In: FUXE, K., Borroto-Escuela, D. (eds) Receptor-Receptor Interactions in the Central Nervous System. Neuromethods, vol 140. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8576-0_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8576-0_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8575-3

  • Online ISBN: 978-1-4939-8576-0

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