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Encapsulation of Active Enzymes within Bacteriophage P22 Virus-Like Particles

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Protein Scaffolds

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1798))

Abstract

Virus-like particles (VLPs) are nonpathogenic protein cage structures derived from viral coat proteins that have found utility in the area of biomaterials and nanotechnology. VLPs have been exploited as containers for the sequestration and encapsulation of a wide range of guest molecules in their hollow interiors. The robust nature of VLPs lend them as versatile scaffolds that can be exploited to provide protection to encapsulated guest molecules, such as enzymes which are often susceptible to inactivation and degradation, and for organization and construction of new nanomaterials incorporating the chemical properties of the guest molecules. In this chapter a background and methodology for the encapsulation of enzymes on the interior of the bacteriophage P22 derived VLP is described.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, The University of Texas at Tyler, Tyler, TX, USA

    Dustin P. Patterson

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  1. Dustin P. Patterson
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Correspondence to Dustin P. Patterson .

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Editors and Affiliations

  1. Department of Chemistry, Occidental College, Los Angeles, California, USA

    Andrew K. Udit

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Patterson, D.P. (2018). Encapsulation of Active Enzymes within Bacteriophage P22 Virus-Like Particles. In: Udit, A. (eds) Protein Scaffolds. Methods in Molecular Biology, vol 1798. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7893-9_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7893-9_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7892-2

  • Online ISBN: 978-1-4939-7893-9

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