Abstract
The utility of reporter genes has gained significant momentum over the last three decades. Reporter genes are used to understand the transcriptional activity of a gene both in vitro and in vivo, and in pathway analysis and drug screening for diseases involving protozoan parasites, and in anti-cancer drug developments. Here, using a human prostate cancer xenograft model (PC3), we describe a method to construct and validate hypoxia reporter genes with different half-lives. Using molecular biology and optical imaging techniques, we have validated the expression of long half-life enhanced green fluorescence protein (EGFP) expression and short half-life luciferase gene expression to report on the spatial and temporal evolution of hypoxia in vivo.
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Acknowledgments
This work was supported by National Institutes of Health R01 CA73850, R01 CA82337, and P50 CA103175.
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Krishnamachary, B., Danhier, P., Kakkad, S., Bharti, S.K., Bhujwalla, Z.M. (2018). Hypoxia-Induced Reporter Genes with Different Half-Lives. In: Dubey, P. (eds) Reporter Gene Imaging. Methods in Molecular Biology, vol 1790. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7860-1_9
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DOI: https://doi.org/10.1007/978-1-4939-7860-1_9
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