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Plant Vacuolar Trafficking

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Cláudia Pereira

    1. Faculty of Sciences, University of Porto, Porto, Portugal

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential from reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1789)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. GREEN FLUORESCENT SEED, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds

    • Tomoo Shimada, Kentaro Fuji, Takuji Ichino, Ooi-Kock Teh, Yasuko Koumoto, Ikuko Hara-Nishimura
    Pages 1-7

  3. Detection of CRISPR/Cas9-Induced Genomic Fragment Deletions in Barley and Generation of Homozygous Edited Lines via Embryogenic Pollen Culture

    • Eszter Kapusi, Eva Stöger
    Pages 9-20

  4. Vacuolar Sorting Determinants: Isolation and Study

    • Bruno Peixoto, Susana Pereira, Cláudia Pereira, José Pissarra
    Pages 21-31

  5. Vacuole-Targeted Proteins: Ins and Outs of Subcellular Localization Studies

    • Inês Carqueijeiro, Liuda J. Sepúlveda, Angela Mosquera, Richard Payne, Cyrielle Corbin, Nicolas Papon et al.
    Pages 33-54

  6. Plant Cell Vacuoles: Staining and Fluorescent Probes

    • Giovanni Stefano, Luciana Renna, Federica Brandizzi
    Pages 55-63

  7. Vacuolar Targeting and Characterization of Recombinant Antibodies

    • Carolina Gabriela Ocampo, Silvana Petruccelli
    Pages 65-80

  8. Isolation of Vacuoles from the Leaves of the Medicinal Plant Catharanthus roseus

    • Inês Carqueijeiro, Henrique Noronha, Sara Bettencourt, Joana G. Guedes, Patrícia Duarte, Hernâni Gerós et al.
    Pages 81-99

  9. Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells

    • Jorge M. P. Rodrigues, Cátia S. Pereira, Natacha Fontes, Hernâni Gerós, Manuela Côrte-Real
    Pages 101-115

  10. Live Cell Imaging and Confocal Microscopy

    • Luciana Renna, Giovanni Stefano, Federica Brandizzi
    Pages 117-130

  11. Imaging Vacuolar Anthocyanins with Fluorescence Lifetime Microscopy (FLIM)

    • Alexandra Chanoca, Brian Burkel, Erich Grotewold, Kevin W. Eliceiri, Marisa S. Otegui
    Pages 131-141

  12. The Use of Drugs in the Study of Vacuole Morphology and Trafficking to the Vacuole in Arabidopsis thaliana

    • Ricardo Tejos, Claudio Osorio-Navarro, Lorena Norambuena
    Pages 143-154

  13. Use of Brefeldin A and Wortmannin to Dissect Post-Golgi Organelles Related to Vacuolar Transport in Arabidopsis thaliana

    • Junpei Takagi, Tomohiro Uemura
    Pages 155-165

  14. Use Endosidin2 to Study Plant Exocytosis and Vacuolar Trafficking

    • Lei Huang, Chunhua Zhang
    Pages 167-175

  15. Visualization of RMRs (Receptor Membrane RING-H2) Dimerization in Nicotiana benthamiana Leaves Using a Bimolecular Fluorescence Complementation (BiFC) Assay

    • Alessandro Occhialini
    Pages 177-194

  16. Vacuole Dynamics in Rice Cells Invaded by the Blast Fungus Magnaporthe oryzae

    • Kiersun Jones, Chang Hyun Khang
    Pages 195-203

  17. Characterization of Plant Glycoproteins: Analysis of Plant Glycopeptide Mass Spectrometry Data with plantGlycoMS, a Package in the R Statistical Computing Environment

    • Margaret R. Baker, Travers Ching, David L. Tabb, Qing X. Li
    Pages 205-220

  18. Back Matter

    Pages 221-222
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Keywords

About this book

This volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a  highly valuable resource for researchers interested in learning more about this field

Editors and Affiliations

  • Faculty of Sciences, University of Porto, Porto, Portugal

    Cláudia Pereira

Bibliographic Information

  • Book Title: Plant Vacuolar Trafficking

  • Book Subtitle: Methods and Protocols

  • Editors: Cláudia Pereira

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7856-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7855-7Published: 19 June 2018

  • Softcover ISBN: 978-1-4939-9305-5Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7856-4Published: 18 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 222

  • Number of Illustrations: 10 b/w illustrations, 41 illustrations in colour

  • Topics: Plant Sciences

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