Abstract
Multi-parametric flow cytometry of tumor-bearing murine nonlymphoid tissue allows for characterization, isolation, and examination of immune cell composition, phenotype, and function. Here we describe an approach to process nonlymphoid tissues and then utilize a base antibody panel to define all of the major immune cell types in a single staining condition. This panel can be used to phenotype tumor-associated macrophages.
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Acknowledgments
The work is supported by NIH grants K08 HL121185, Mandel Fellowship Award, and Proof of Concept Award from the Pulmonary Hypertension Association.
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Ian Cumming, R., Yu, YR.A. (2018). Phenotyping Tumor-Associated Macrophages. In: Rousselet, G. (eds) Macrophages. Methods in Molecular Biology, vol 1784. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7837-3_10
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DOI: https://doi.org/10.1007/978-1-4939-7837-3_10
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7836-6
Online ISBN: 978-1-4939-7837-3
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