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High Throughput Sequencing-Based Approaches for Gene Expression Analysis

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Gene Expression Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1783))

Abstract

Next-generation sequencing has emerged as the method of choice to answer fundamental questions in biology. The massively parallel sequencing technology for RNA-Seq analysis enables better understanding of gene expression patterns in model and nonmodel organisms. Sequencing per se has reached the stage of commodity level while analyzing and interpreting huge amount of data has been a significant challenge. This chapter is aimed at discussing the complexities involved in sequencing and analysis, and tries to simplify sequencing based gene expression analysis. Biologists and experimental scientists were kept in mind while discussing the methods and analysis workflow.

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Author information

Authors and Affiliations

  1. Clevergene Biocorp Private Limited, Bangalore, Karnataka, India

    R. Raja Sekhara Reddy & M. V. Ramanujam

Authors
  1. R. Raja Sekhara Reddy
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  2. M. V. Ramanujam
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Editor information

Editors and Affiliations

  1. Division of Geriatrics and Clinical Gerontology, National Institute on Aging, Bethesda, Maryland, USA

    Nalini Raghavachari

  2. Environmental Laboratory, US Army Engineer Research and Development Center, Vicksburg, Mississippi, USA

    Natàlia Garcia-Reyero

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Reddy, R.R.S., Ramanujam, M.V. (2018). High Throughput Sequencing-Based Approaches for Gene Expression Analysis. In: Raghavachari, N., Garcia-Reyero, N. (eds) Gene Expression Analysis. Methods in Molecular Biology, vol 1783. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7834-2_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7834-2_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7833-5

  • Online ISBN: 978-1-4939-7834-2

  • eBook Packages: Springer Protocols

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