Abstract
Natural killer (NK) cells are an essential component of innate immunity. These lymphocytes are also sensitive barometers of the effects of endogenous and exogenous stressors on the immune system. This chapter describes a chromium (51Cr)-release bioassay designed to measure to the target cell killing capacity of NK cells (NKCC). Key features of the cytotoxicity assay are that it is done with whole blood and that numbers of effector cells are determined for each sample by flow cytometry and lymphocyte count. Effector cells are defined as CD3-CD56+ lymphocytes. Target cells are the K562 erythroleukemia cell line. Killing capacity is defined as number of target cells killed per effector cell, at an effector cell/target cell ratio of 1:1 during a 4-h in vitro assay.
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Acknowledgments
This work was supported by grants from the NIAAA: R21AA016635 (PI MA Fletcher); NIAID: R01AI065723 (PI MA Fletcher); CFIDS Assoc. of America: (PI N Klimas); NIAID: UO1 AI459940 (PI N Klimas); NIAMSD R01 AR057853-01A1 (PI N Klimas); VA merit awards (PI N Klimas) Dynamic Modeling in GWI, CFS/ME; and GW080152 (PI N Klimas).
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Fletcher, M.A., Barnes, Z., Broderick, G., Klimas, N.G. (2018). Psychoneuroimmunology and Natural Killer Cells: The Chromium-Release Whole-Blood Assay. In: Yan, Q. (eds) Psychoneuroimmunology. Methods in Molecular Biology, vol 1781. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7828-1_12
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DOI: https://doi.org/10.1007/978-1-4939-7828-1_12
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