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Production and Use of Recombinant Aβ for Aggregation Studies

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Peptide Self-Assembly

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1777))

Abstract

The amyloid β-protein (Aβ) is believed to play a central role in Alzheimer’s disease (AD) pathogenesis and there is great interest in understanding the process of Aβ aggregation, its underlying mechanism and the species generated during aggregation and their biological activity. Although Aβ has been studied for more than 30 years, analysis of its aggregation has been hampered by structural and chemical impurities. Here we provide a detailed protocol for the expression and purification of chemically and structurally homogeneous Aβ monomer. We also describe a method to produce covalent Aβ dimers linked by phenolic coupling of tyrosine residues.

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Acknowledgments

This work was supported by grants to DMW from the National Institute of Health (AG046275) and the BrightFocus Foundation and to SL from the European Research Council and the Swedish Research Council. We thank Dr. Gunnar Brinkmalm for mass spec. analysis.

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Authors and Affiliations

  1. Laboratory for Neurodegenerative Research, Center for Neurologic Diseases, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA

    Tiernan T. O’Malley & Dominic M. Walsh

  2. Department of Biochemistry and Structural Biology, Lund University, Lund, Sweden

    Sara Linse

Authors
  1. Tiernan T. O’Malley
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  2. Sara Linse
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  3. Dominic M. Walsh
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Corresponding author

Correspondence to Dominic M. Walsh .

Editor information

Editors and Affiliations

  1. Department of Chemistry, University of Rochester, Rochester, New York, USA

    Bradley L. Nilsson

  2. Department of Medicinal Chemistry, Institute for Translational Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA

    Todd M. Doran

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O’Malley, T.T., Linse, S., Walsh, D.M. (2018). Production and Use of Recombinant Aβ for Aggregation Studies. In: Nilsson, B., Doran, T. (eds) Peptide Self-Assembly. Methods in Molecular Biology, vol 1777. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7811-3_19

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  • DOI: https://doi.org/10.1007/978-1-4939-7811-3_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7809-0

  • Online ISBN: 978-1-4939-7811-3

  • eBook Packages: Springer Protocols

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