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3D Clearing and Molecular Labeling in Plant Tissues

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Photosynthesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1770))

Abstract

Plant histology and imaging traditionally involve the transformation of tissues into thin sections to minimize light scatter in opaque material, allowing optical clarity and high-resolution microscopy. Recently, new techniques in 3D tissue clearing, including PEA-CLARITY, have been developed to minimize light scatter within intact, whole samples. These techniques can achieve equivalent microscopic resolution to that of thin section imaging with the added benefit of maintaining the original 3D structure and position of biomolecules of interest. Furthermore, PEA-CLARITY is compatible with standard stains and immunohistochemistry, allowing molecular interrogation of intact, 3D tissues. This chapter outlines the current methods available for 3D histology in plants and details the materials, equipment, reagents, and procedure for the PEA-CLARITY technique.

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Author information

Authors and Affiliations

  1. School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia

    William M. Palmer, Antony P. Martin, Stephanie L. Reed & Christopher P. L. Grof

  2. School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, Australia

    Jamie R. Flynn

  3. CSIRO Agriculture, Black Mountain, ACT, Australia

    Rosemary G. White

  4. ARC Centre of Excellence for Translational Photosynthesis, Australian National University, Acton, ACT, Australia

    Robert T. Furbank

Authors
  1. William M. Palmer
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  2. Jamie R. Flynn
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  3. Antony P. Martin
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  4. Stephanie L. Reed
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  5. Christopher P. L. Grof
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  6. Rosemary G. White
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  7. Robert T. Furbank
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Editor information

Editors and Affiliations

  1. Niceville, Florida, USA

    Sarah Covshoff

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Palmer, W.M. et al. (2018). 3D Clearing and Molecular Labeling in Plant Tissues. In: Covshoff, S. (eds) Photosynthesis. Methods in Molecular Biology, vol 1770. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7786-4_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7786-4_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7785-7

  • Online ISBN: 978-1-4939-7786-4

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