Skip to main content
SpringerLink
Log in

Virus Elimination Through Meristem Culture and Rapid Clonal Propagation Using a Temporary Immersion System

  • Protocol
  • First Online:
Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

The generation of virus-free plants is a key requirement in the international trade of orchid seedlings. Shoot apical meristem isolation and its successful culture is one of the methods that can generate virus-free plants. In this chapter, a general protocol for meristem isolation and subsequent micropropagation of meristem explants and regenerated plantlets for Phalaenopsis is detailed. Patience, practice, and cleanliness are key elements in shoot apical meristem excision. Information on the temporary immersion system in the mass propagation of virus-free Phalaenopsis plantlets is also presented.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 219.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 279.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Chang CA (2006) Diagnosis, appraisal, and detection of orchids virus. Symposium of health inspection, quarantine diagnosis, and detective technology of plant disease. Vol V. Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture, Executive Yuan. Taiwan; p. 103–118

    Google Scholar 

  2. Yam TW, Arditti J (2009) History of orchid propagation: a mirror of the history of biotechnology. Plant Biotechnol Rep 3:1–56

    Article  Google Scholar 

  3. Morel G, Martin C (1952) Guerison de dahlias atteints dune maladie a virus. Comptes rendus hebdomadaires des seances de l academie des. Sciences 235:1324–1325

    CAS  Google Scholar 

  4. Morel G (1960) Producing virus-free Cymbidiums. Amer Orchid Soc Bull 29:495–497

    Google Scholar 

  5. Nehra NS, Kartha KK (1994) Meristem and shoot tip culture: requirements and applications. In: Vasil IK, Thorpe TA (eds) Plant cell and tissue culture. Kluwer, Dordrecht, pp 37–70

    Google Scholar 

  6. Panattoni A, Luvisi A, Triolo E (2013) Review. Elimination of viruses in plants: twenty years of progress. Span J Agric Res 11:173–188

    Article  Google Scholar 

  7. Meri K, Hosokawa D (1977) Localization of viruses in apical meristem and production of virus-free plant by means of meristem and tissue culture. Acta Hort 78:389–397

    Article  Google Scholar 

  8. Grout BWW (1990) Meristem-tip culture for propagation and virus elimination. In: Hall RD (ed) Plant cell culture protocols. Humana Press, Totowa, NJ, pp 115–125

    Google Scholar 

  9. Kane ME (2005) Shoot culture procedures. In: Trigiano RN (ed) Plant development and biotechnology. CRC Press, Boca Raton, FL, pp 145–157

    Google Scholar 

  10. Novak FJ, Maskova I (1979) Apical shoot tip culture of tomato. Sci Hortic 10:337–344

    Article  CAS  Google Scholar 

  11. Quak F (1977) Meristem culture and virus-free plants. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin, Heidelberg/New York, pp 598–615

    Google Scholar 

  12. Vasil IK, Thorpe TA (eds) (1994) Plant cell and tissue culture. Kluwer, Dordrecht

    Google Scholar 

  13. George EF, Hall MA, De Klerk GJ (2007) Plant propagation by tissue culture 3rd edition: volume 1. The background. Exegetic, Basingstoke

    Google Scholar 

  14. Trigiano RN, Gray DJ (2000) Plant tissue culture: concepts and laboratory exercises, 2nd edn. CRC Press, Boca Raton, FL

    Google Scholar 

  15. Cutter EG (1971) Plant anatomy. Experiment and interpretation. Part 2. Organs. Edward Arnold, London

    Google Scholar 

  16. Kumar S, Kan MS, Raj SK, Sharma AK (2009) Elimination of mixed infection of Cucumber mosaic and Tomato aspermy virus from Chrysanthemum morifolium Ramat. cv. Pooja by shoot meristem culture. Sci Hortic 119:108–112

    Article  CAS  Google Scholar 

  17. Wang Q, Valkonen JPT (2008) Elimination of two viruses which interact synergistically from sweet potato by shoot tip culture and cryotherapy. J Virol Methods 154:135–145

    Article  CAS  Google Scholar 

  18. Verbeek M, Dijk P, Well PMA (1995) Efficiency of eradication of four viruses from garlic (Allium sativum) by meristem-tip culture. Eur J Plant Pathol 101:231–239

    Article  Google Scholar 

  19. Kang F-U, Hsu S-T, Shen R-S (2011) Virus elimination through meristem culture and rapid clonal propagation by temporary immersion system in Phalaenopsis. J Taiwan Soc Hort Sci 57:207–218

    Google Scholar 

  20. Santa Cruz S (1999) Perspective: phloem transport of viruses and macromolecules- what goes in must come out. Trends Microbiol 7:237–241

    Article  CAS  Google Scholar 

  21. Moreno IM, Thompsont JR, Garcia-Arenal F (2004) Analysis of the systemic colonization of cucumber plants by Cucumber green mottle mosaic virus. J Gen Virol 85:749–759

    Article  CAS  Google Scholar 

  22. Opalka N, Brugidou C, Bonneau C, Nicole M, Beachy RN, Yeager M, Fauquet C (1998) Movement of rice yellow mottle virus between xylem cells through pit membranes. Proc Natl Acad Sci 95:3323–3328

    Article  CAS  Google Scholar 

  23. Carrington JC, Kasschau KD, Mahajan SK, Schaad MC (1996) Cell-to-cell and long-distance transport of viruses in plants. Plant Cell 8:1669–1681

    Article  CAS  Google Scholar 

  24. Hsieh SC (2006) Studies on the virus-elimination techniques of green onion ‘Lan Yang No. 1’and ‘Tao Yuan No. 3’. MS thesis. National Chung Hsing University Taichung, Taiwan

    Google Scholar 

  25. Hull R (2002) Mathews’ plant virology, 4th edn. Academic Press, Cambridge, MA

    Google Scholar 

  26. Pennazio S, Roggero P (1996) Plant hormones and plant virus diseases. The auxins. New Microbiol 19:369–378

    CAS  PubMed  Google Scholar 

  27. Lim ST, Wong SM, Goh CJ (1993) Elimination of Cymbidium mosaic virus and Odontoglossum ringspot virus from orchids by meristem culture and thin section culture with chemotherapy. Ann Appl Biol 122:289–297

    Article  Google Scholar 

  28. Loi JS, Lee SM, Lam-Chan LT, Fan S, Yiang WH (1991) Eradication of orchid viruses in Dendrobium Sonia using virazole. Singapore J Primary Ind 19:16–22

    Google Scholar 

  29. Chien K-W, Agrawal DC, Tsay H-S, Chang C-A (2015) Elimination of mixed ‘Odontoglossum ring sport’ and ‘Cymbidium mosaic’ viruses from Phalaenopsis hybrid ‘V3’ through shoot-tip culture and protocorm-like body selection. Crop Prot 67:1–6

    Article  Google Scholar 

  30. Park SY, Yeung EC, Chakrabarty D, Paek K (2002) An efficient direct induction of protocorm-like bodies from leaf subepidermal cells of Doritaenopsis hybrid using thin-section culture. Plant Cell Rep 21:46–51

    Article  CAS  Google Scholar 

  31. Etienne H, Berthouly M (2002) Temporary immersion systems in plant micropropagation. Plant Cell Tiss Org Cult 69:215–231

    Article  Google Scholar 

  32. Watt MP (2012) The status of temporary immersion system (TIS) technology for plant micropropagation. Afr J Biotechnol 11:14025–14035

    CAS  Google Scholar 

  33. Georgiev V, Schumann A, Pavlov A, Bley T (2014) Temporary immersion systems in plant biotechnology. Eng Life Sci 14:607–621

    Article  CAS  Google Scholar 

  34. Shen TM, Shen RS, Chan SI (2004) Protocorm like bodies induction of Phalaenopsis hybrids using short-stems derived in vitro shoots as explants. APOC 8:293–307

    Google Scholar 

  35. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–479

    Article  CAS  Google Scholar 

  36. Kang FY (2012) Virus elimination through shoot apical meristem, protocorm-like bodies culture and rapid clonal propagation by temporary immersion system in Phalaenopsis. MS thesis. National Chiayi University, Chiayi, Taiwan

    Google Scholar 

  37. Stasolla C, Yeung EC (2015) Paraffin and polyester waxes. In: Yeung ECT, Stasolla C, Sumner MJ, Huang BQ (eds) Plant microtechniques and protocols. Springer, New York, pp 45–66

    Chapter  Google Scholar 

  38. Teng WL, Sin T, Teng MC (2002) Explant preparation affects culture initiation and regeneration of Panax ginseng and Panax quinquefolius. Plant Cell Tiss Org Cult 68:233–239

    Article  CAS  Google Scholar 

  39. Prommee P (2009) Establishment of regeneration system via axillary bud culture in Phalaenopsis by temporary immersion system. MS thesis, Department of Horticulture National Chiayi University, Chiayi, Taiwan, ROC

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Horticultural Science, National Chiayi University, Chiayi City, Taiwan, Republic of China

    Rong-Show Shen & Shan-Te Hsu

Authors
  1. Rong-Show Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shan-Te Hsu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rong-Show Shen .

Editor information

Editors and Affiliations

  1. Biology Department, National Museum of Natural Science, Taichung, China

    Yung-I Lee

  2. Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada

    Edward Chee-Tak Yeung

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Shen, RS., Hsu, ST. (2018). Virus Elimination Through Meristem Culture and Rapid Clonal Propagation Using a Temporary Immersion System. In: Lee, YI., Yeung, ET. (eds) Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols. Springer Protocols Handbooks. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7771-0_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7771-0_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7770-3

  • Online ISBN: 978-1-4939-7771-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation