Abstract
Peptide self-assembly is an important field in biomaterials in which short peptides are designed to aggregate into nanostructures that often form hydrogels. These peptides are typically made using solid-phase peptide synthesis (SPPS), a technique in which amino acids are added sequentially to a growing chain. This technique has been used to synthesize peptides with more than 100 amino acids. However, self-assembling peptides are designed to aggregate in solution, which often reduces coupling efficiency during synthesis and makes purification more difficult. Here, an outline of solid-phase peptide synthesis is provided, along with steps that can be used to improve the synthetic yield and purification of self-assembling peptides for regenerative medicine applications.
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Thomas Pashuck, E. (2018). Synthesis of Self-Assembling Peptide-Based Hydrogels for Regenerative Medicine Using Solid-Phase Peptide Synthesis. In: Chawla, K. (eds) Biomaterials for Tissue Engineering. Methods in Molecular Biology, vol 1758. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7741-3_14
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DOI: https://doi.org/10.1007/978-1-4939-7741-3_14
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7741-3
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