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Immunohistochemical Phenotyping of Mouse Amacrine Cell Subtypes

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Mouse Retinal Phenotyping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1753))

Abstract

The retina shares its embryological origin with the central nervous system (CNS), so the neural circuitry of the retina has long been considered to be a relatively simple model of the neural networks in the brain, sharing similar morphologies, neurotransmitters, and receptors. Amacrine cells are, by far, the largest group of inhibitory neurons in the retina that also have the most diverse range of phenotypes of any retinal neuron. Here, I describe an approach, using immunolabeling of cryosections, to identify different subclasses of amacrine cell in the mouse retina.

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Acknowledgment

This work was supported by a grant from the National Eye Research Centre in the UK (RJ6042).

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Correspondence to Denize Atan .

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Atan, D. (2018). Immunohistochemical Phenotyping of Mouse Amacrine Cell Subtypes. In: Tanimoto, N. (eds) Mouse Retinal Phenotyping. Methods in Molecular Biology, vol 1753. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7720-8_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7720-8_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7719-2

  • Online ISBN: 978-1-4939-7720-8

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