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Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice

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Sertoli Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1748))

Abstract

Testis development and function is regulated by intricate cell-cell cross talk. Characterization of the mechanisms underpinning this has been derived through a wide variety of approaches including pharmacological manipulation, transgenics, and cell-specific ablation of populations. The removal of all or a proportion of a specific cell type has been achieved through a variety of approaches. In this paper, we detail a combined transgenic and pharmacological approach to ablate the Sertoli or germ cell populations using diphtheria toxin in mice. We describe the key steps in generation, validation, and use of the models and also describe the caveats and cautions necessary. We also provide a detailed description of the methodology applied to characterize testis development and function in models of postnatal Sertoli or germ cell ablation.

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Acknowledgments

We thank Lyndsey Cruickshanks, Nathan Jeffery, Sarah Smith, Laura Milne, Laura O’Hara, Yi Ting Tsai, Rod Mitchell, Mike Millar, Forbes Howie, Mike Dodds, and Ana Monteiro for technical support. The work described in this article was funded by a Biotechnology and Biological Sciences Research Council (BBSRC) Project Grant [BB/J015105/1 to L.B.S., P.O’S.] and a Medical Research Council (MRC) Programme Grant [MR/N002970/1 to L.B.S.].

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Authors and Affiliations

  1. MRC Centre for Reproductive Health, The Queen’s Medical Research Institute, University of Edinburgh, Edinburgh, UK

    Diane Rebourcet, Annalucia Darbey, Michael Curley & Lee B. Smith

  2. Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Glasgow, UK

    Diane Rebourcet & Peter O’Shaughnessy

  3. School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia

    Lee B. Smith

Authors
  1. Diane Rebourcet
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  2. Annalucia Darbey
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  3. Michael Curley
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  4. Peter O’Shaughnessy
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  5. Lee B. Smith
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Corresponding author

Correspondence to Lee B. Smith .

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Editors and Affiliations

  1. Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal

    Marco G. Alves

  2. Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal

    Pedro F. Oliveira

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Rebourcet, D., Darbey, A., Curley, M., O’Shaughnessy, P., Smith, L.B. (2018). Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice. In: Alves, M., Oliveira, P. (eds) Sertoli Cells. Methods in Molecular Biology, vol 1748. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7698-0_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7698-0_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7697-3

  • Online ISBN: 978-1-4939-7698-0

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