Abstract
Here we describe a protocol to utilize human benign leiomyoma tissue in in vitro 3D model that enables an assessment of cell invasion. The chapter also describes detailed instructions for image analysis to quantify the results. Leiomyoma is a benign tumor of the uterus which mimics authentic components of the tumor microenvironment including fibroblasts, vessels, collagen fibers, and extracellular protein composition. The leiomyoma invasion model represents a superior 3D model for cell invasion studies compared to the other non-human organotypic models.
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Benton G, Arnaoutova I, George J et al (2014) Matrigel: from discovery and ECM mimicry to assays and models for cancer research. Adv Drug Deliv Rev 79-80:3–18
Salo T, Sutinen M, al HAE e (2015) A novel human leiomyoma tissue derived matrix for cell culture studies. BMC Cancer 15:981
Teppo S, Sundquist E, Vered M et al (2013) The hypoxic tumor microenvironment regulates invasion of aggressive oral carcinoma cells. Exp Cell Res 319(4):376–389
Nurmenniemi S, Sinikumpu T, Alahuhta I et al (2009) A novel organotypic model mimics the tumor microenvironment. Am J Pathol 175(3):1281–1291
Sundquist E, Renko O, Salo S et al (2016) Neoplastic extracellular matrix environment promotes cancer invasion in vitro. Exp Cell Res 344(2):229–240
Alahuhta I, Aikio M, Väyrynen O et al (2015) Endostatin induces proliferation of oral carcinoma cells but its effect on invasion is modified by the tumor microenvironment. Exp Cell Res 336:130–140
Schindelin J, Arganda-Carreras I, Frise E et al (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9(7):676–682
Acknowledgments
This work was supported by Sigrid Juselius Foundation, the Finnish Cancer Foundation, the University of Helsinki Central Hospital, and the Health Science Council of the Academy of Finland (Centre of Excellence 2012–2017 Grant 251314). We thank Dr. Sini Nurmenniemi and Dr. Tatu Pirkola for developing the myoma model and the protocol of invasion quantification, respectively. We thank Mr. Donald Smart for the editorial assistance.
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Åström, P., Heljasvaara, R., Nyberg, P., Al-Samadi, A., Salo, T. (2018). Human Tumor Tissue-Based 3D In Vitro Invasion Assays. In: Cal, S., Obaya, A. (eds) Proteases and Cancer. Methods in Molecular Biology, vol 1731. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7595-2_19
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DOI: https://doi.org/10.1007/978-1-4939-7595-2_19
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7594-5
Online ISBN: 978-1-4939-7595-2
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